A tuneable array of unique steady-state microfluidic gradients

Matthew D. Estes, Cedric Hurth, Matthew Barrett, Frederic Zenhausern

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


We report an on-chip gradient generator that has been designed, modelled, fabricated, and characterized to facilitate temporal tuning of several unique gradients in parallel for multiple applications. This design allows for steady state programming of the intensities across multiple orders of magnitude while producing exponential, linear, and logarithmic gradient profiles. The magnitude of the gradients is controlled through regulating the ratio of the two on-chip flow inlets without the need for valves or other active mixers. On-chip binding of biotin by a fluorescent streptavidin complex creates a diffusive barrier that regulates access to the gradient inlets, providing a second orthogonal mechanism for regulating the microgradient intensities. The device is also characterized using an on-chip enzymatic reaction to produce an array of tuneable product concentrations within the various microchannels.

Original languageEnglish (US)
Pages (from-to)12805-12814
Number of pages10
JournalPhysical Chemistry Chemical Physics
Issue number31
StatePublished - Aug 21 2013

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry


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