A study on proliferation and phenotypical stability of schwann cell on Keratin/PLGA film

A. Young Oh; Soon Hee Kim; Yun Tae Kim; Na Ri Jeon; Jae Chan Yang; Sang Jin Lee; James J. Yoo; Mark Van Dyke; Hyung Sik Shin; John M. Rhee; Gilson Khang

A study on proliferation and phenotypical stability of schwann cell on Keratin/PLGA film

A. Young Oh, Soon Hee Kim, Yun Tae Kim, Na Ri Jeon, Jae Chan Yang, Sang Jin Lee, James J. Yoo, Mark Van Dyke, Hyung Sik Shin, John M. Rhee, Gilson Khang

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Keratin contains regulatory molecules that can enhance neuronal cell activity. We fabricated keratin/ PLGA films using 0, 10, 20, and 50 wt% of keratin using solvent casting method. We measured the contact angle of each film and cell proliferation was assayed by counting the cells attached on the film. Adhered cell morphology was confirmed by scanning electron microscope. RT-PCR was conducted to evaluate the gene expression of NF, NSE, and S-100, the Schwann cell markers. The keratin content of 20 and 50 wt% provided higher wettability than PLGA. The 20 wt% keratin was better in cell adhesion and proliferation of SCs than other keratin/PLGA films. The phenotypic stability of SC was maintained with the keratin content of 10 and 20 wt%.

Original language	English (US)
Pages (from-to)	118-123
Number of pages	6
Journal	Polymer (Korea)
Volume	33
Issue number	2
State	Published - Mar 2009
Externally published	Yes

Keywords

Keratin
PLGA
Phenotype
Proliferation
Schwann cell

ASJC Scopus subject areas

General Chemical Engineering
Polymers and Plastics
Materials Chemistry

Cite this

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title = "A study on proliferation and phenotypical stability of schwann cell on Keratin/PLGA film",

abstract = "Keratin contains regulatory molecules that can enhance neuronal cell activity. We fabricated keratin/ PLGA films using 0, 10, 20, and 50 wt% of keratin using solvent casting method. We measured the contact angle of each film and cell proliferation was assayed by counting the cells attached on the film. Adhered cell morphology was confirmed by scanning electron microscope. RT-PCR was conducted to evaluate the gene expression of NF, NSE, and S-100, the Schwann cell markers. The keratin content of 20 and 50 wt% provided higher wettability than PLGA. The 20 wt% keratin was better in cell adhesion and proliferation of SCs than other keratin/PLGA films. The phenotypic stability of SC was maintained with the keratin content of 10 and 20 wt%.",

keywords = "Keratin, PLGA, Phenotype, Proliferation, Schwann cell",

author = "Oh, {A. Young} and Kim, {Soon Hee} and Kim, {Yun Tae} and Jeon, {Na Ri} and Yang, {Jae Chan} and Lee, {Sang Jin} and Yoo, {James J.} and {Van Dyke}, Mark and Shin, {Hyung Sik} and Rhee, {John M.} and Gilson Khang",

year = "2009",

month = mar,

language = "English (US)",

volume = "33",

pages = "118--123",

journal = "Polymer (Korea)",

issn = "0379-153X",

publisher = "Polymer Society of Korea",

number = "2",

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TY - JOUR

T1 - A study on proliferation and phenotypical stability of schwann cell on Keratin/PLGA film

AU - Oh, A. Young

AU - Kim, Soon Hee

AU - Kim, Yun Tae

AU - Jeon, Na Ri

AU - Yang, Jae Chan

AU - Lee, Sang Jin

AU - Yoo, James J.

AU - Van Dyke, Mark

AU - Shin, Hyung Sik

AU - Rhee, John M.

AU - Khang, Gilson

PY - 2009/3

Y1 - 2009/3

N2 - Keratin contains regulatory molecules that can enhance neuronal cell activity. We fabricated keratin/ PLGA films using 0, 10, 20, and 50 wt% of keratin using solvent casting method. We measured the contact angle of each film and cell proliferation was assayed by counting the cells attached on the film. Adhered cell morphology was confirmed by scanning electron microscope. RT-PCR was conducted to evaluate the gene expression of NF, NSE, and S-100, the Schwann cell markers. The keratin content of 20 and 50 wt% provided higher wettability than PLGA. The 20 wt% keratin was better in cell adhesion and proliferation of SCs than other keratin/PLGA films. The phenotypic stability of SC was maintained with the keratin content of 10 and 20 wt%.

AB - Keratin contains regulatory molecules that can enhance neuronal cell activity. We fabricated keratin/ PLGA films using 0, 10, 20, and 50 wt% of keratin using solvent casting method. We measured the contact angle of each film and cell proliferation was assayed by counting the cells attached on the film. Adhered cell morphology was confirmed by scanning electron microscope. RT-PCR was conducted to evaluate the gene expression of NF, NSE, and S-100, the Schwann cell markers. The keratin content of 20 and 50 wt% provided higher wettability than PLGA. The 20 wt% keratin was better in cell adhesion and proliferation of SCs than other keratin/PLGA films. The phenotypic stability of SC was maintained with the keratin content of 10 and 20 wt%.

KW - Keratin

KW - PLGA

KW - Phenotype

KW - Proliferation

KW - Schwann cell

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M3 - Article

AN - SCOPUS:64249115464

SN - 0379-153X

VL - 33

SP - 118

EP - 123

JO - Polymer (Korea)

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IS - 2

ER -

A study on proliferation and phenotypical stability of schwann cell on Keratin/PLGA film

Abstract

Keywords

ASJC Scopus subject areas

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