A Study of Lipid Biosynthesis in Cryptococcus Neoformans

Ineko Inoue; Fariba Mirbod; Mariko Seishima; Shunji Mori; Yoshinori Nozawa

doi:10.3314/jjmm.35.421

A Study of Lipid Biosynthesis in Cryptococcus Neoformans

Ineko Inoue, Fariba Mirbod, Mariko Seishima, Shunji Mori, Yoshinori Nozawa

Research output: Contribution to journal › Article › peer-review

Abstract

Changes in incorporation of [¹⁴C] acetate into lipid fractions were examined in Cryptococcus neoformans during growth and were compared with those in Candida albicans. The uptake of [¹⁴C] acetate in the total lipids of Cr. neoformans gradually increased following the growth phase and then decreased at the stationary phase. The hydrolysis of triacylglycerol (TG), the main lipid in Cr. neoformans, was considered to be accelerated as the radioactivity in TG decreased with a concomitant increase of radioactivity in free fatty acid (FFA) in the stationary phase. In contrast, in C. albicans, phosphatidylcholine (PC), the major component of cell membrane, increased during the growth phase and decreased in the stationary phase. It was also observed that the biosynthesis of acidic phospholipids, PI and PS, was enhanced during the growth phase in both types of fungi.

Original language	English (US)
Pages (from-to)	421-427
Number of pages	7
Journal	Nippon Ishinkin Gakkai Zasshi
Volume	35
Issue number	4
DOIs	https://doi.org/10.3314/jjmm.35.421
State	Published - 1994
Externally published	Yes

Keywords

Candida albicans
Cryptococcus neoformans
lipid biosynthesis

ASJC Scopus subject areas

Microbiology
Infectious Diseases

Access to Document

10.3314/jjmm.35.421

Cite this

@article{6b3d539f372547ea9eb21ca2c0654989,

title = "A Study of Lipid Biosynthesis in Cryptococcus Neoformans",

abstract = "Changes in incorporation of [14C] acetate into lipid fractions were examined in Cryptococcus neoformans during growth and were compared with those in Candida albicans. The uptake of [14C] acetate in the total lipids of Cr. neoformans gradually increased following the growth phase and then decreased at the stationary phase. The hydrolysis of triacylglycerol (TG), the main lipid in Cr. neoformans, was considered to be accelerated as the radioactivity in TG decreased with a concomitant increase of radioactivity in free fatty acid (FFA) in the stationary phase. In contrast, in C. albicans, phosphatidylcholine (PC), the major component of cell membrane, increased during the growth phase and decreased in the stationary phase. It was also observed that the biosynthesis of acidic phospholipids, PI and PS, was enhanced during the growth phase in both types of fungi.",

keywords = "Candida albicans, Cryptococcus neoformans, lipid biosynthesis",

author = "Ineko Inoue and Fariba Mirbod and Mariko Seishima and Shunji Mori and Yoshinori Nozawa",

year = "1994",

doi = "10.3314/jjmm.35.421",

language = "English (US)",

volume = "35",

pages = "421--427",

journal = "Nippon Ishinkin Gakkai Zasshi",

issn = "0916-4804",

publisher = "Japanese Society for Medical Mycology",

number = "4",

}

TY - JOUR

T1 - A Study of Lipid Biosynthesis in Cryptococcus Neoformans

AU - Inoue, Ineko

AU - Mirbod, Fariba

AU - Seishima, Mariko

AU - Mori, Shunji

AU - Nozawa, Yoshinori

PY - 1994

Y1 - 1994

N2 - Changes in incorporation of [14C] acetate into lipid fractions were examined in Cryptococcus neoformans during growth and were compared with those in Candida albicans. The uptake of [14C] acetate in the total lipids of Cr. neoformans gradually increased following the growth phase and then decreased at the stationary phase. The hydrolysis of triacylglycerol (TG), the main lipid in Cr. neoformans, was considered to be accelerated as the radioactivity in TG decreased with a concomitant increase of radioactivity in free fatty acid (FFA) in the stationary phase. In contrast, in C. albicans, phosphatidylcholine (PC), the major component of cell membrane, increased during the growth phase and decreased in the stationary phase. It was also observed that the biosynthesis of acidic phospholipids, PI and PS, was enhanced during the growth phase in both types of fungi.

AB - Changes in incorporation of [14C] acetate into lipid fractions were examined in Cryptococcus neoformans during growth and were compared with those in Candida albicans. The uptake of [14C] acetate in the total lipids of Cr. neoformans gradually increased following the growth phase and then decreased at the stationary phase. The hydrolysis of triacylglycerol (TG), the main lipid in Cr. neoformans, was considered to be accelerated as the radioactivity in TG decreased with a concomitant increase of radioactivity in free fatty acid (FFA) in the stationary phase. In contrast, in C. albicans, phosphatidylcholine (PC), the major component of cell membrane, increased during the growth phase and decreased in the stationary phase. It was also observed that the biosynthesis of acidic phospholipids, PI and PS, was enhanced during the growth phase in both types of fungi.

KW - Candida albicans

KW - Cryptococcus neoformans

KW - lipid biosynthesis

UR - http://www.scopus.com/inward/record.url?scp=85007770722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007770722&partnerID=8YFLogxK

U2 - 10.3314/jjmm.35.421

DO - 10.3314/jjmm.35.421

M3 - Article

AN - SCOPUS:85007770722

SN - 0916-4804

VL - 35

SP - 421

EP - 427

JO - Nippon Ishinkin Gakkai Zasshi

JF - Nippon Ishinkin Gakkai Zasshi

IS - 4

ER -

A Study of Lipid Biosynthesis in Cryptococcus Neoformans

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this