A spin filter method for continuous evaluation of hemolysis

Fernando Alvarez-Núñez; Danielle A. Rosin; Samuel H. Yalkowsky

doi:10.1021/js980484l

A spin filter method for continuous evaluation of hemolysis

Fernando Alvarez-Núñez, Danielle A. Rosin, Samuel H. Yalkowsky

Pharmacology and Toxicology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A novel method for quantifying hemolysis is described. This method uses a spin filter to separate the free hemoglobin from the red blood cells suspended in the test solution. This procedure enables the use of a closed loop system that continuously measures hemolysis spectrophotometrically. It is shown that hemolysis does not always stop after the solution has been quenched with normal saline. In fact, the process of hemolysis induced by chemicals such as potassium oleate is relatively slow.

Original language	English (US)
Pages (from-to)	1041-1043
Number of pages	3
Journal	Journal of pharmaceutical sciences
Volume	88
Issue number	10
DOIs	https://doi.org/10.1021/js980484l
State	Published - Oct 1999

ASJC Scopus subject areas

Pharmaceutical Science

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10.1021/js980484l

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@article{794ddb57f7034ba1b87193eeefb06eb7,

title = "A spin filter method for continuous evaluation of hemolysis",

abstract = "A novel method for quantifying hemolysis is described. This method uses a spin filter to separate the free hemoglobin from the red blood cells suspended in the test solution. This procedure enables the use of a closed loop system that continuously measures hemolysis spectrophotometrically. It is shown that hemolysis does not always stop after the solution has been quenched with normal saline. In fact, the process of hemolysis induced by chemicals such as potassium oleate is relatively slow.",

author = "Fernando Alvarez-N{\'u}{\~n}ez and Rosin, {Danielle A.} and Yalkowsky, {Samuel H.}",

note = "Funding Information: We thank The Pharmaceutical Research and Manufactures of America Foundation (PhRMA) for their generous support through the 1997 Undergraduate Research Fellowship. The work of Ms. Mi Jin Kim is appreciated. Also, we thank CONACYT and UNAM of M{\'e}xico.",

year = "1999",

month = oct,

doi = "10.1021/js980484l",

language = "English (US)",

volume = "88",

pages = "1041--1043",

journal = "Journal of pharmaceutical sciences",

issn = "0022-3549",

publisher = "Elsevier B.V.",

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T1 - A spin filter method for continuous evaluation of hemolysis

AU - Alvarez-Núñez, Fernando

AU - Rosin, Danielle A.

AU - Yalkowsky, Samuel H.

N1 - Funding Information: We thank The Pharmaceutical Research and Manufactures of America Foundation (PhRMA) for their generous support through the 1997 Undergraduate Research Fellowship. The work of Ms. Mi Jin Kim is appreciated. Also, we thank CONACYT and UNAM of México.

PY - 1999/10

Y1 - 1999/10

N2 - A novel method for quantifying hemolysis is described. This method uses a spin filter to separate the free hemoglobin from the red blood cells suspended in the test solution. This procedure enables the use of a closed loop system that continuously measures hemolysis spectrophotometrically. It is shown that hemolysis does not always stop after the solution has been quenched with normal saline. In fact, the process of hemolysis induced by chemicals such as potassium oleate is relatively slow.

AB - A novel method for quantifying hemolysis is described. This method uses a spin filter to separate the free hemoglobin from the red blood cells suspended in the test solution. This procedure enables the use of a closed loop system that continuously measures hemolysis spectrophotometrically. It is shown that hemolysis does not always stop after the solution has been quenched with normal saline. In fact, the process of hemolysis induced by chemicals such as potassium oleate is relatively slow.

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ER -

A spin filter method for continuous evaluation of hemolysis

Abstract

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