TY - JOUR
T1 - A specific role for the TFIID subunit TAF4 and RanBPM in neural progenitor differentiation
AU - Brunkhorst, Adrian
AU - Karlén, Mattias
AU - Shi, Jiaqi
AU - Mikolajczyk, Monika
AU - Nelson, Mark A.
AU - Metsis, Madis
AU - Hermanson, Ola
N1 - Funding Information:
The authors are grateful for the support and assistance from Drs Christer Höög, Irwin Davidson, Johan Ericson, and Tamas Bartfai. We further like to thank Drs. Josh Duckworth, Ana Teixeira, Karolina Wallenborg and members of the Hermanson lab for technical assistance and comments on the manuscript. Supported by grants from NCI (CA 70145, M.A.N.), the Swedish Cancer Foundation, the Swedish Research Council (M.M. and O.H.), the Swedish Children's Cancer Society, the Magn. Bergvall Foundation, the Lars Hierta Foundation, the Jeansson Foundation, the Thuring Foundation, the Åke Wiberg Foundation, the Åhlén Foundation, the Swedish Medical Society, and the Swedish Foundation for Strategic Research (O.H.).
PY - 2005/6
Y1 - 2005/6
N2 - TAF4 is crucial for the activity of many transcription factors, including CREB, RAR and CSL/RBP-Jκ, but the role for TAF4 in neural development is unknown. Embryonic cortical neural stem cells (NSC) showed strong expression of TAF4 that decreased during neuronal but not glial differentiation. In a protein-protein interaction screen, we identified the intracellular signaling factor RanBPM as a co-factor of TAF4. RanBPM co-localized with TAF4 in a subset of mitotic progenitors in vivo and endogenous TAF4 and RanBPM could be co-immunoprecipitated from NSC extracts. Interestingly, co-transfections of TAF4 and RanBPM led to a significant increase in the number of primary neurite processes but no increase in total neurite length, whereas RanBPM and a TAF4 isoform lacking the RanBPM-interacting domain exerted no significant effect. Our results demonstrate that temporally high expression levels of two factors considered to be relatively general in function can influence very specific events in neuronal differentiation.
AB - TAF4 is crucial for the activity of many transcription factors, including CREB, RAR and CSL/RBP-Jκ, but the role for TAF4 in neural development is unknown. Embryonic cortical neural stem cells (NSC) showed strong expression of TAF4 that decreased during neuronal but not glial differentiation. In a protein-protein interaction screen, we identified the intracellular signaling factor RanBPM as a co-factor of TAF4. RanBPM co-localized with TAF4 in a subset of mitotic progenitors in vivo and endogenous TAF4 and RanBPM could be co-immunoprecipitated from NSC extracts. Interestingly, co-transfections of TAF4 and RanBPM led to a significant increase in the number of primary neurite processes but no increase in total neurite length, whereas RanBPM and a TAF4 isoform lacking the RanBPM-interacting domain exerted no significant effect. Our results demonstrate that temporally high expression levels of two factors considered to be relatively general in function can influence very specific events in neuronal differentiation.
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U2 - 10.1016/j.mcn.2005.02.015
DO - 10.1016/j.mcn.2005.02.015
M3 - Article
C2 - 15911349
AN - SCOPUS:19444377299
SN - 1044-7431
VL - 29
SP - 250
EP - 258
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 2
ER -