A single bout of dynamic exercise by healthy adults enhances the generation of monocyte-derived-dendritic cells

Emily C.P. LaVoy, Catherine M. Bollard, Patrick J. Hanley, Daniel P. O'Connor, Thomas W. Lowder, Jos A. Bosch, Richard J. Simpson

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The ex vivo generation of monocyte-derived-dendritic cells (mo-DCs) has facilitated the use of DCs in immunotherapy research. However, low blood monocyte numbers frequently limit the manufacture of sufficient numbers of mo-DCs for subsequent experimental and clinical procedures. Because exercise mobilizes monocytes to the blood, we tested if acute dynamic exercise by healthy adults would augment the generation of mo-DCs without compromising their differentiation or function. We compared mo-DC generation from before- and after-exercise blood over 8-days of culture. Function was assessed by FITC-dextran uptake and the stimulation of autologous cytomegalovirus (pp65)-specific-T-cells. Supporting the hypothesis, we found a near fourfold increase in number of mo-DCs generated after-exercise. Furthermore, relative FITC-dextran uptake, differentiation rate, and stimulation of pp65-specific-T-cells did not differ between before- and after-exercise mo-DCs. We conclude that exercise enhances the ex vivo generation of mo-DCs without compromising their function, and so may overcome some limitations associated with manufacturing these cells for immunotherapy.

Original languageEnglish (US)
Pages (from-to)52-59
Number of pages8
JournalCellular Immunology
Volume295
Issue number1
DOIs
StatePublished - May 1 2015
Externally publishedYes

Keywords

  • Dendritic cells
  • Immunology
  • Immunotherapy
  • Physical activity

ASJC Scopus subject areas

  • Immunology

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