A simple method for isolation of megabase DNA from cotton

Xinping Zhao; Hong Bin Zhang; Rod A. Wing; Andrew H. Paterson

doi:10.1007/BF02668372

A simple method for isolation of megabase DNA from cotton

Xinping Zhao
, Hong Bin Zhang
, Rod A. Wing
, Andrew H. Paterson

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning.

Original language	English (US)
Pages (from-to)	110-115
Number of pages	6
Journal	Plant Molecular Biology Reporter
Volume	12
Issue number	2
DOIs	https://doi.org/10.1007/BF02668372
State	Published - Jun 1994
Externally published	Yes

Keywords

Gossypium
genome analysis
high molecular weight DNA
physical mapping
pulsed-field gel electrophoresis

ASJC Scopus subject areas

Molecular Biology
Plant Science

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10.1007/BF02668372

Cite this

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title = "A simple method for isolation of megabase DNA from cotton",

abstract = "A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning.",

keywords = "Gossypium, genome analysis, high molecular weight DNA, physical mapping, pulsed-field gel electrophoresis",

author = "Xinping Zhao and Zhang, \{Hong Bin\} and Wing, \{Rod A.\} and Paterson, \{Andrew H.\}",

year = "1994",

month = jun,

doi = "10.1007/BF02668372",

language = "English (US)",

volume = "12",

pages = "110--115",

journal = "Plant Molecular Biology Reporter",

issn = "0735-9640",

publisher = "Springer",

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AU - Zhao, Xinping

AU - Zhang, Hong Bin

AU - Wing, Rod A.

AU - Paterson, Andrew H.

PY - 1994/6

Y1 - 1994/6

N2 - A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning.

AB - A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning.

KW - Gossypium

KW - genome analysis

KW - high molecular weight DNA

KW - physical mapping

KW - pulsed-field gel electrophoresis

UR - https://www.scopus.com/pages/publications/51249168476

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U2 - 10.1007/BF02668372

DO - 10.1007/BF02668372

M3 - Article

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SN - 0735-9640

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JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

IS - 2

ER -

A simple method for isolation of megabase DNA from cotton

Abstract

Keywords

ASJC Scopus subject areas

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