A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

S. R. Farrah; S. M. Goyal; C. P. Gerba; R. H. Conklin; C. Wallis; J. L. Melnick; H. L. DuPont

doi:10.1159/000148921

A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

S. R. Farrah, S. M. Goyal, C. P. Gerba, R. H. Conklin, C. Wallis, J. L. Melnick, H. L. DuPont

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Organic compounds in cell culture harvests known as membrane coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy fiberglass filters. Subsequent elution with pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100 fold.

Original language	English (US)
Pages (from-to)	56-59
Number of pages	4
Journal	Unknown Journal
Volume	9
Issue number	1
DOIs	https://doi.org/10.1159/000148921
State	Published - 1978
Externally published	Yes

ASJC Scopus subject areas

Virology
Infectious Diseases

Access to Document

10.1159/000148921

Cite this

@article{cdc16dc6e6ed43c99897d7237d46d3b2,

title = "A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters",

abstract = "Organic compounds in cell culture harvests known as membrane coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy fiberglass filters. Subsequent elution with pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100 fold.",

author = "Farrah, {S. R.} and Goyal, {S. M.} and Gerba, {C. P.} and Conklin, {R. H.} and C. Wallis and Melnick, {J. L.} and DuPont, {H. L.}",

year = "1978",

doi = "10.1159/000148921",

language = "English (US)",

volume = "9",

pages = "56--59",

journal = "Unknown Journal",

issn = "0375-9474",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

AU - Farrah, S. R.

AU - Goyal, S. M.

AU - Gerba, C. P.

AU - Conklin, R. H.

AU - Wallis, C.

AU - Melnick, J. L.

AU - DuPont, H. L.

PY - 1978

Y1 - 1978

N2 - Organic compounds in cell culture harvests known as membrane coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy fiberglass filters. Subsequent elution with pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100 fold.

AB - Organic compounds in cell culture harvests known as membrane coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy fiberglass filters. Subsequent elution with pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100 fold.

UR - http://www.scopus.com/inward/record.url?scp=0017913427&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017913427&partnerID=8YFLogxK

U2 - 10.1159/000148921

DO - 10.1159/000148921

M3 - Article

C2 - 202572

AN - SCOPUS:0017913427

SN - 0375-9474

VL - 9

SP - 56

EP - 59

JO - Unknown Journal

JF - Unknown Journal

IS - 1

ER -

A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this