Abstract
A serum-free medium has been devised that supports the proliferation and differentiation of primary cultures of rat skeletal muscle satellite cells for up to 4 d. The medium consists of a mixture of Dulbecco's modified Eagle's medium and MCDB-104 plus insulin, dexamethasone, pituitary fibroblast growth factor, Deutsch fetuin, and linoleic acid. In addition to promoting the formation of myotubes from satellite cells, a decrease in fibroblast contamination of these cultures was observed when cultures grown in serum-free medium were compared to cultures grown in serum-containing medium.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 636-640 |
| Number of pages | 5 |
| Journal | In Vitro Cellular & Developmental Biology |
| Volume | 21 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1985 |
Keywords
- fetuin
- fibroblast growth factor
- insulin
- myogenesis
- satellite cell
- serum-free
ASJC Scopus subject areas
- Biotechnology
- Plant Science
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