A serum-free medium that supports the growth of cultured skeletal muscle satellite cells

Ronald E. Allen; Michael V. Dodson; Lynda S. Luiten; Linda K. Boxhorn

doi:10.1007/BF02623296

A serum-free medium that supports the growth of cultured skeletal muscle satellite cells

Ronald E. Allen, Michael V. Dodson, Lynda S. Luiten, Linda K. Boxhorn

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

A serum-free medium has been devised that supports the proliferation and differentiation of primary cultures of rat skeletal muscle satellite cells for up to 4 d. The medium consists of a mixture of Dulbecco's modified Eagle's medium and MCDB-104 plus insulin, dexamethasone, pituitary fibroblast growth factor, Deutsch fetuin, and linoleic acid. In addition to promoting the formation of myotubes from satellite cells, a decrease in fibroblast contamination of these cultures was observed when cultures grown in serum-free medium were compared to cultures grown in serum-containing medium.

Original language	English (US)
Pages (from-to)	636-640
Number of pages	5
Journal	In Vitro Cellular & Developmental Biology
Volume	21
Issue number	11
DOIs	https://doi.org/10.1007/BF02623296
State	Published - Nov 1985

Keywords

fetuin
fibroblast growth factor
insulin
myogenesis
satellite cell
serum-free

ASJC Scopus subject areas

Biotechnology
Plant Science

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10.1007/BF02623296

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title = "A serum-free medium that supports the growth of cultured skeletal muscle satellite cells",

abstract = "A serum-free medium has been devised that supports the proliferation and differentiation of primary cultures of rat skeletal muscle satellite cells for up to 4 d. The medium consists of a mixture of Dulbecco's modified Eagle's medium and MCDB-104 plus insulin, dexamethasone, pituitary fibroblast growth factor, Deutsch fetuin, and linoleic acid. In addition to promoting the formation of myotubes from satellite cells, a decrease in fibroblast contamination of these cultures was observed when cultures grown in serum-free medium were compared to cultures grown in serum-containing medium.",

keywords = "fetuin, fibroblast growth factor, insulin, myogenesis, satellite cell, serum-free",

author = "Allen, \{Ronald E.\} and Dodson, \{Michael V.\} and Luiten, \{Lynda S.\} and Boxhorn, \{Linda K.\}",

year = "1985",

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doi = "10.1007/BF02623296",

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T1 - A serum-free medium that supports the growth of cultured skeletal muscle satellite cells

AU - Allen, Ronald E.

AU - Dodson, Michael V.

AU - Luiten, Lynda S.

AU - Boxhorn, Linda K.

PY - 1985/11

Y1 - 1985/11

N2 - A serum-free medium has been devised that supports the proliferation and differentiation of primary cultures of rat skeletal muscle satellite cells for up to 4 d. The medium consists of a mixture of Dulbecco's modified Eagle's medium and MCDB-104 plus insulin, dexamethasone, pituitary fibroblast growth factor, Deutsch fetuin, and linoleic acid. In addition to promoting the formation of myotubes from satellite cells, a decrease in fibroblast contamination of these cultures was observed when cultures grown in serum-free medium were compared to cultures grown in serum-containing medium.

AB - A serum-free medium has been devised that supports the proliferation and differentiation of primary cultures of rat skeletal muscle satellite cells for up to 4 d. The medium consists of a mixture of Dulbecco's modified Eagle's medium and MCDB-104 plus insulin, dexamethasone, pituitary fibroblast growth factor, Deutsch fetuin, and linoleic acid. In addition to promoting the formation of myotubes from satellite cells, a decrease in fibroblast contamination of these cultures was observed when cultures grown in serum-free medium were compared to cultures grown in serum-containing medium.

KW - fetuin

KW - fibroblast growth factor

KW - insulin

KW - myogenesis

KW - satellite cell

KW - serum-free

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JO - In Vitro Cellular & Developmental Biology

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IS - 11

ER -

A serum-free medium that supports the growth of cultured skeletal muscle satellite cells

Abstract

Keywords

ASJC Scopus subject areas

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