A serum-free medium that supports the growth of cultured skeletal muscle satellite cells

Ronald E. Allen, Michael V. Dodson, Lynda S. Luiten, Linda K. Boxhorn

    Research output: Contribution to journalArticlepeer-review

    50 Scopus citations

    Abstract

    A serum-free medium has been devised that supports the proliferation and differentiation of primary cultures of rat skeletal muscle satellite cells for up to 4 d. The medium consists of a mixture of Dulbecco's modified Eagle's medium and MCDB-104 plus insulin, dexamethasone, pituitary fibroblast growth factor, Deutsch fetuin, and linoleic acid. In addition to promoting the formation of myotubes from satellite cells, a decrease in fibroblast contamination of these cultures was observed when cultures grown in serum-free medium were compared to cultures grown in serum-containing medium.

    Original languageEnglish (US)
    Pages (from-to)636-640
    Number of pages5
    JournalIn Vitro Cellular & Developmental Biology
    Volume21
    Issue number11
    DOIs
    StatePublished - Nov 1985

    Keywords

    • fetuin
    • fibroblast growth factor
    • insulin
    • myogenesis
    • satellite cell
    • serum-free

    ASJC Scopus subject areas

    • Biotechnology
    • Plant Science

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