A sensitive radioenzymatic assay for norepinephrine in tissues and plasma

David P. Henry; Barbra J. Starman; David G. Johnson; Robert H. Williams

doi:10.1016/0024-3205(75)90258-1

A sensitive radioenzymatic assay for norepinephrine in tissues and plasma

David P. Henry, Barbra J. Starman, David G. Johnson, Robert H. Williams

Medicine

Research output: Contribution to journal › Article › peer-review

414 Scopus citations

Abstract

A procedure for measuring picogram quantities of norepinephrine has been developed utilizing partially purified bovine adrenal phenylethanolamine-N-methyl-transferase and ³[H]-S-adenosyl-methionine. The sensitivity of the assay is 25 pg, and the procedure is applicable to many body tissues and fluids, including brain and plasma.

Original language	English (US)
Pages (from-to)	375-384
Number of pages	10
Journal	Life Sciences
Volume	16
Issue number	3
DOIs	https://doi.org/10.1016/0024-3205(75)90258-1
State	Published - Feb 1 1975

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(75)90258-1

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title = "A sensitive radioenzymatic assay for norepinephrine in tissues and plasma",

abstract = "A procedure for measuring picogram quantities of norepinephrine has been developed utilizing partially purified bovine adrenal phenylethanolamine-N-methyl-transferase and 3[H]-S-adenosyl-methionine. The sensitivity of the assay is 25 pg, and the procedure is applicable to many body tissues and fluids, including brain and plasma.",

author = "Henry, {David P.} and Starman, {Barbra J.} and Johnson, {David G.} and Williams, {Robert H.}",

note = "Funding Information: This research was supported by NIH research grants AM 05020-19 and AM Funding Information: Johnson is tha recipient of a NIH Career Development Award (K04 AM 70727) .",

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T1 - A sensitive radioenzymatic assay for norepinephrine in tissues and plasma

AU - Henry, David P.

AU - Starman, Barbra J.

AU - Johnson, David G.

AU - Williams, Robert H.

N1 - Funding Information: This research was supported by NIH research grants AM 05020-19 and AM Funding Information: Johnson is tha recipient of a NIH Career Development Award (K04 AM 70727) .

PY - 1975/2/1

Y1 - 1975/2/1

N2 - A procedure for measuring picogram quantities of norepinephrine has been developed utilizing partially purified bovine adrenal phenylethanolamine-N-methyl-transferase and 3[H]-S-adenosyl-methionine. The sensitivity of the assay is 25 pg, and the procedure is applicable to many body tissues and fluids, including brain and plasma.

AB - A procedure for measuring picogram quantities of norepinephrine has been developed utilizing partially purified bovine adrenal phenylethanolamine-N-methyl-transferase and 3[H]-S-adenosyl-methionine. The sensitivity of the assay is 25 pg, and the procedure is applicable to many body tissues and fluids, including brain and plasma.

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ER -

A sensitive radioenzymatic assay for norepinephrine in tissues and plasma

Abstract

ASJC Scopus subject areas

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