A sensitive microassay for the murine alternative compliment pathway

Keith A. Joiner; Robert Shahon; Jeffrey A. Gelfand

doi:10.1016/0022-1759(79)90141-8

A sensitive microassay for the murine alternative compliment pathway

Keith A. Joiner, Robert Shahon, Jeffrey A. Gelfand

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A microhemolytic assay for measurement of murine alternative complement pathway activity is described. The assay uses ⁵¹Cr release from neuraminidase-treated rabbit erythrocytes incubated with Mg²⁺ EGTA-chelated murine serum. Neuraminidase pretreatment of rabbit erythrocytes increases the sensitivity of the assay 8-10-fold, enabling the use of small volumes of individual mouse sera. The assay affords a simple, sensitive and reproducible method for measuring murine alternative complement pathway activity. Significant differences were found between strains when alternative complement pathway activity of serum from various inbred murine lines was measured.

Original language	English (US)
Pages (from-to)	283-290
Number of pages	8
Journal	Journal of Immunological Methods
Volume	31
Issue number	3-4
DOIs	https://doi.org/10.1016/0022-1759(79)90141-8
State	Published - Dec 27 1979
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1016/0022-1759(79)90141-8

Cite this

@article{e791d15c7fa448fa95aff03573cce6d7,

title = "A sensitive microassay for the murine alternative compliment pathway",

abstract = "A microhemolytic assay for measurement of murine alternative complement pathway activity is described. The assay uses 51Cr release from neuraminidase-treated rabbit erythrocytes incubated with Mg2+ EGTA-chelated murine serum. Neuraminidase pretreatment of rabbit erythrocytes increases the sensitivity of the assay 8-10-fold, enabling the use of small volumes of individual mouse sera. The assay affords a simple, sensitive and reproducible method for measuring murine alternative complement pathway activity. Significant differences were found between strains when alternative complement pathway activity of serum from various inbred murine lines was measured.",

author = "Joiner, {Keith A.} and Robert Shahon and Gelfand, {Jeffrey A.}",

year = "1979",

month = dec,

day = "27",

doi = "10.1016/0022-1759(79)90141-8",

language = "English (US)",

volume = "31",

pages = "283--290",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "3-4",

}

TY - JOUR

T1 - A sensitive microassay for the murine alternative compliment pathway

AU - Joiner, Keith A.

AU - Shahon, Robert

AU - Gelfand, Jeffrey A.

PY - 1979/12/27

Y1 - 1979/12/27

N2 - A microhemolytic assay for measurement of murine alternative complement pathway activity is described. The assay uses 51Cr release from neuraminidase-treated rabbit erythrocytes incubated with Mg2+ EGTA-chelated murine serum. Neuraminidase pretreatment of rabbit erythrocytes increases the sensitivity of the assay 8-10-fold, enabling the use of small volumes of individual mouse sera. The assay affords a simple, sensitive and reproducible method for measuring murine alternative complement pathway activity. Significant differences were found between strains when alternative complement pathway activity of serum from various inbred murine lines was measured.

AB - A microhemolytic assay for measurement of murine alternative complement pathway activity is described. The assay uses 51Cr release from neuraminidase-treated rabbit erythrocytes incubated with Mg2+ EGTA-chelated murine serum. Neuraminidase pretreatment of rabbit erythrocytes increases the sensitivity of the assay 8-10-fold, enabling the use of small volumes of individual mouse sera. The assay affords a simple, sensitive and reproducible method for measuring murine alternative complement pathway activity. Significant differences were found between strains when alternative complement pathway activity of serum from various inbred murine lines was measured.

UR - http://www.scopus.com/inward/record.url?scp=0018627377&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018627377&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(79)90141-8

DO - 10.1016/0022-1759(79)90141-8

M3 - Article

C2 - 521632

AN - SCOPUS:0018627377

SN - 0022-1759

VL - 31

SP - 283

EP - 290

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 3-4

ER -

A sensitive microassay for the murine alternative compliment pathway

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this