@article{9cbc707834684e69b9d471ee37ebece6,
title = "A route to de novo domestication of wild allotetraploid rice",
abstract = "Cultivated rice varieties are all diploid, and polyploidization of rice has long been desired because of its advantages in genome buffering, vigorousness, and environmental robustness. However, a workable route remains elusive. Here, we describe a practical strategy, namely de novo domestication of wild allotetraploid rice. By screening allotetraploid wild rice inventory, we identified one genotype of Oryza alta (CCDD), polyploid rice 1 (PPR1), and established two important resources for its de novo domestication: (1) an efficient tissue culture, transformation, and genome editing system and (2) a high-quality genome assembly discriminated into two subgenomes of 12 chromosomes apiece. With these resources, we show that six agronomically important traits could be rapidly improved by editing O. alta homologs of the genes controlling these traits in diploid rice. Our results demonstrate the possibility that de novo domesticated allotetraploid rice can be developed into a new staple cereal to strengthen world food security. Li and colleagues developed a breeding route to de novo domestication of wild allotetraploid rice that provides a rational strategy for creating novel crops and generated a series of allotetraploid rice lines edited in domestication-related and agronomically important genes.",
keywords = "Oryza alta, comparative genomics, de novo domestication, genetic transformation, genome, genome editing, genome evolution, polyploid, structural variation, tissue culture",
author = "Hong Yu and Tao Lin and Xiangbing Meng and Huilong Du and Jingkun Zhang and Guifu Liu and Mingjiang Chen and Yanhui Jing and Liquan Kou and Xiuxiu Li and Qiang Gao and Yan Liang and Xiangdong Liu and Zhilan Fan and Yuntao Liang and Zhukuan Cheng and Mingsheng Chen and Zhixi Tian and Yonghong Wang and Chengcai Chu and Jianru Zuo and Jianmin Wan and Qian Qian and Bin Han and Andrea Zuccolo and Wing, {Rod A.} and Caixia Gao and Chengzhi Liang and Jiayang Li",
note = "Funding Information: We thank Prof. Yaoguang Liu (South China Agricultural University) for providing pYLCRISPR/Cas9P ubi -H vector. The research was supported by the National Natural Science Foundation of China ( 31788103 ), the Chinese Academy of Sciences ( XDA24030504 and XDA24040201 ), the National Key Research and Development Program of China ( 2016YFD0101800 ), and the National Transgenic Science and Technology Program of China ( 2019ZX08010-003 and 2019ZX08010-001 ). Funding Information: We thank Prof. Yaoguang Liu (South China Agricultural University) for providing pYLCRISPR/Cas9Pubi-H vector. The research was supported by the National Natural Science Foundation of China (31788103), the Chinese Academy of Sciences (XDA24030504 and XDA24040201), the National Key Research and Development Program of China (2016YFD0101800), and the National Transgenic Science and Technology Program of China (2019ZX08010-003 and 2019ZX08010-001). H.Y. T.L. X.M. H.D. J. Zhang, L.K. X. Li, Q.G. M.C. G.L. Y.J. and Yan Liang performed the experiments. H.Y. T.L. X.M. H.D. J. Zhang, Z.T. C.C. J. Zuo, Y.W. C.G. Q.Q. B.H. C.L. and J.L. analyzed the data. Z.F. Yuntao Liang, Z.C. M.C. X. Liu, and J.W. collected and provided germplasm. H.Y. C.G. C.L. and J.L. designed the experiments. J.L. conceived the project. H.Y. T.L. and J.L. wrote the manuscript with input from C.G. C.L. J. Zhang, J. Zuo, X.M. A.Z. and R.W. All authors have read, edited, and approved the content of the manuscript. C.G. is a member of the Cell advisory board. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = mar,
day = "4",
doi = "10.1016/j.cell.2021.01.013",
language = "English (US)",
volume = "184",
pages = "1156--1170.e14",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",
}