A review of nasopharyngeal swab and saliva tests for SARS-CoV-2 infection: Disease timelines, relative sensitivities, and test optimization

Marylin Roque; Kevin Proudfoot; Vadim Mathys; Sophie Yu; Natalie Krieger; Thomas Gernon; Kash Gokli; Stanley Hamilton; Colin Cook; Yuman Fong

doi:10.1002/jso.26561

A review of nasopharyngeal swab and saliva tests for SARS-CoV-2 infection: Disease timelines, relative sensitivities, and test optimization

Marylin Roque
, Kevin Proudfoot
, Vadim Mathys
, Sophie Yu
, Natalie Krieger
, Thomas Gernon
, Kash Gokli
, Stanley Hamilton
, Colin Cook
, Yuman Fong

Surgery

Research output: Contribution to journal › Review article › peer-review

16 Scopus citations

Abstract

Testing is an essential part of containment of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. This review summarizes studies for SARS-CoV-2 infection and testing. Nasopharyngeal samples are best at sensitivity detection, especially in early stages of disease and in asymptomatic individuals. Current swab processing involves a 100- to 1000-fold dilution of the patient sample. Future optimization of testing should focus on using smaller volumes of viral transport media and swab designs to increase comfort and increased viral adhesion.

Original language	English (US)
Pages (from-to)	465-475
Number of pages	11
Journal	Journal of Surgical Oncology
Volume	124
Issue number	4
DOIs	https://doi.org/10.1002/jso.26561
State	Published - Sep 15 2021

Keywords

COVID-19
bronchitis
pharyngitis
pneumonia
respiratory infection

ASJC Scopus subject areas

Surgery
Oncology

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10.1002/jso.26561

Cite this

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title = "A review of nasopharyngeal swab and saliva tests for SARS-CoV-2 infection: Disease timelines, relative sensitivities, and test optimization",

abstract = "Testing is an essential part of containment of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. This review summarizes studies for SARS-CoV-2 infection and testing. Nasopharyngeal samples are best at sensitivity detection, especially in early stages of disease and in asymptomatic individuals. Current swab processing involves a 100- to 1000-fold dilution of the patient sample. Future optimization of testing should focus on using smaller volumes of viral transport media and swab designs to increase comfort and increased viral adhesion.",

keywords = "COVID-19, bronchitis, pharyngitis, pneumonia, respiratory infection",

author = "Marylin Roque and Kevin Proudfoot and Vadim Mathys and Sophie Yu and Natalie Krieger and Thomas Gernon and Kash Gokli and Stanley Hamilton and Colin Cook and Yuman Fong",

note = "Funding Information: The authors would like to acknowledge and thank several individuals whose contributions have made this paper possible. The authors thank Tyler Seto, Vice President of Enterprise Quality and Patient Safety at City of Hope, for explaining how healthcare professionals are trained and how COVID-19 tests are administered at a testing site. We would also like to express our gratitude toward Gilbert Morales, supervisor of Clinical Pathology from City of Hope, for providing essential information on how testing samples are processed within the lab. Finally, we would like to thank Vivian Lam, Kaitlyn Paulsen, and Supriya Deshpande for providing editing assistance. Publisher Copyright: {\textcopyright} 2021 Wiley Periodicals LLC",

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AU - Krieger, Natalie

AU - Gernon, Thomas

AU - Gokli, Kash

AU - Hamilton, Stanley

AU - Cook, Colin

AU - Fong, Yuman

N1 - Funding Information: The authors would like to acknowledge and thank several individuals whose contributions have made this paper possible. The authors thank Tyler Seto, Vice President of Enterprise Quality and Patient Safety at City of Hope, for explaining how healthcare professionals are trained and how COVID-19 tests are administered at a testing site. We would also like to express our gratitude toward Gilbert Morales, supervisor of Clinical Pathology from City of Hope, for providing essential information on how testing samples are processed within the lab. Finally, we would like to thank Vivian Lam, Kaitlyn Paulsen, and Supriya Deshpande for providing editing assistance. Publisher Copyright: © 2021 Wiley Periodicals LLC

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AB - Testing is an essential part of containment of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. This review summarizes studies for SARS-CoV-2 infection and testing. Nasopharyngeal samples are best at sensitivity detection, especially in early stages of disease and in asymptomatic individuals. Current swab processing involves a 100- to 1000-fold dilution of the patient sample. Future optimization of testing should focus on using smaller volumes of viral transport media and swab designs to increase comfort and increased viral adhesion.

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KW - bronchitis

KW - pharyngitis

KW - pneumonia

KW - respiratory infection

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A review of nasopharyngeal swab and saliva tests for SARS-CoV-2 infection: Disease timelines, relative sensitivities, and test optimization

Abstract

Keywords

ASJC Scopus subject areas

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