A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution

Francisco G. Nobrega; Carol L. Dieckmann; Alexander Tzagoloff

doi:10.1016/0003-2697(83)90145-8

A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution

Francisco G. Nobrega
, Carol L. Dieckmann
, Alexander Tzagoloff

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.

Original language	English (US)
Pages (from-to)	141-145
Number of pages	5
Journal	Analytical Biochemistry
Volume	131
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(83)90145-8
State	Published - May 1983
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(83)90145-8

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title = "A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution",

abstract = "A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.",

author = "Nobrega, \{Francisco G.\} and Dieckmann, \{Carol L.\} and Alexander Tzagoloff",

note = "Funding Information: research was supported by a National Science Grant PCM-8 116680. F.G.N. has been sup-travel grants from FAPESP, CNPq, and FINEP",

year = "1983",

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doi = "10.1016/0003-2697(83)90145-8",

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AU - Nobrega, Francisco G.

AU - Dieckmann, Carol L.

AU - Tzagoloff, Alexander

N1 - Funding Information: research was supported by a National Science Grant PCM-8 116680. F.G.N. has been sup-travel grants from FAPESP, CNPq, and FINEP

PY - 1983/5

Y1 - 1983/5

N2 - A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.

AB - A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.

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U2 - 10.1016/0003-2697(83)90145-8

DO - 10.1016/0003-2697(83)90145-8

M3 - Article

C2 - 6193726

AN - SCOPUS:0020523751

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

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ER -

A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution

Abstract

ASJC Scopus subject areas

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