TY - JOUR
T1 - A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution
AU - Nobrega, Francisco G.
AU - Dieckmann, Carol L.
AU - Tzagoloff, Alexander
N1 - Funding Information:
research was supported by a National Science Grant PCM-8 116680. F.G.N. has been sup-travel grants from FAPESP, CNPq, and FINEP
PY - 1983/5
Y1 - 1983/5
N2 - A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.
AB - A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.
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U2 - 10.1016/0003-2697(83)90145-8
DO - 10.1016/0003-2697(83)90145-8
M3 - Article
C2 - 6193726
AN - SCOPUS:0020523751
SN - 0003-2697
VL - 131
SP - 141
EP - 145
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -