A procedure for increasing the contrast of biological specimens in edge‐projection TEM

J. A. Panitz, D. G. Bear

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Rotary shadowing has been used to increase the image contrast of biological specimens during edge‐projection imaging in the transmission electron microscope (TEM). In this imaging mode, biological specimens are adsorbed from aqueous solution onto a highly curved substrate and observed in a direction parallel to its surface. High contrast TEM images are obtained at 200 kV when a 1–3 nm layer of tungsten is thermally evaporated onto the substrate at an angle of about 9°. Individual adsorbates are clearly delineated by the smooth, continuous, and fine‐grained tungsten layer that surrounds them. TEM images obtained with this technique can provide a unique view of biological adsorbates on metal, insulator or semiconductor substrates. 1985 Blackwell Science Ltd

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalJournal of Microscopy
Volume138
Issue number1
DOIs
StatePublished - Apr 1985
Externally publishedYes

Keywords

  • Rotary shadowing
  • ferritin
  • field emission
  • protein
  • transmission electron microscopy
  • tungsten thin films

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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