A plaque assay for the simian rotavirus SAII

E. M. Smith; M. Kolb Estes; D. Y. Graham; C. P. Gerba

doi:10.1099/0022-1317-43-3-513

A plaque assay for the simian rotavirus SAII

E. M. Smith
, M. Kolb Estes
, D. Y. Graham
, C. P. Gerba

Research output: Contribution to journal › Article › peer-review

93 Scopus citations

Abstract

A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

Original language	English (US)
Pages (from-to)	513-519
Number of pages	7
Journal	Journal of General Virology
Volume	43
Issue number	3
DOIs	https://doi.org/10.1099/0022-1317-43-3-513
State	Published - 1979
Externally published	Yes

ASJC Scopus subject areas

Virology

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10.1099/0022-1317-43-3-513

Cite this

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title = "A plaque assay for the simian rotavirus SAII",

abstract = "A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.",

author = "Smith, \{E. M.\} and \{Kolb Estes\}, M. and Graham, \{D. Y.\} and Gerba, \{C. P.\}",

year = "1979",

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AU - Smith, E. M.

AU - Kolb Estes, M.

AU - Graham, D. Y.

AU - Gerba, C. P.

PY - 1979

Y1 - 1979

N2 - A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

AB - A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

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UR - https://www.scopus.com/pages/publications/0018770376#tab=citedBy

U2 - 10.1099/0022-1317-43-3-513

DO - 10.1099/0022-1317-43-3-513

M3 - Article

C2 - 225432

AN - SCOPUS:0018770376

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SP - 513

EP - 519

JO - Journal of General Virology

JF - Journal of General Virology

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ER -

A plaque assay for the simian rotavirus SAII

Abstract

ASJC Scopus subject areas

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