A plaque assay for the simian rotavirus SAII

E. M. Smith; M. Kolb Estes; D. Y. Graham; C. P. Gerba

doi:10.1099/0022-1317-43-3-513

A plaque assay for the simian rotavirus SAII

E. M. Smith, M. Kolb Estes, D. Y. Graham, C. P. Gerba

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Abstract

A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

Original language	English (US)
Pages (from-to)	513-519
Number of pages	7
Journal	Journal of General Virology
Volume	43
Issue number	3
DOIs	https://doi.org/10.1099/0022-1317-43-3-513
State	Published - 1979
Externally published	Yes

ASJC Scopus subject areas

Virology

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10.1099/0022-1317-43-3-513

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title = "A plaque assay for the simian rotavirus SAII",

abstract = "A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.",

author = "Smith, {E. M.} and {Kolb Estes}, M. and Graham, {D. Y.} and Gerba, {C. P.}",

year = "1979",

doi = "10.1099/0022-1317-43-3-513",

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AU - Smith, E. M.

AU - Kolb Estes, M.

AU - Graham, D. Y.

AU - Gerba, C. P.

PY - 1979

Y1 - 1979

N2 - A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

AB - A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

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A plaque assay for the simian rotavirus SAII

Abstract

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