A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis

Wanjin Xing; Hang Xu; Huijuan Ma; Syed Ali Abbas Abedi; Shanshan Wang; Xingchen Zhang; Xiaogang Liu; Huan Xu; Wei Wang; Kaiyan Lou

doi:10.1039/d1cc06611k

A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis

Wanjin Xing
, Hang Xu
, Huijuan Ma
, Syed Ali Abbas Abedi
, Shanshan Wang
, Xingchen Zhang
, Xiaogang Liu
, Huan Xu
, Wei Wang
, Kaiyan Lou

Pharmacology and Toxicology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A fluorescent probe (COU-LIP-1) for monitoring labile Fe(ii) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(ii)-induced reductive cleavage of the N-O bond results in a turn-on response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(ii) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.

Original language	English (US)
Pages (from-to)	2979-2982
Number of pages	4
Journal	Chemical Communications
Volume	58
Issue number	18
DOIs	https://doi.org/10.1039/d1cc06611k
State	Published - Mar 4 2022

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis",

abstract = "A fluorescent probe (COU-LIP-1) for monitoring labile Fe(ii) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(ii)-induced reductive cleavage of the N-O bond results in a turn-on response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(ii) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.",

author = "Wanjin Xing and Hang Xu and Huijuan Ma and Abedi, \{Syed Ali Abbas\} and Shanshan Wang and Xingchen Zhang and Xiaogang Liu and Huan Xu and Wei Wang and Kaiyan Lou",

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T1 - A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis

AU - Xing, Wanjin

AU - Xu, Hang

AU - Ma, Huijuan

AU - Abedi, Syed Ali Abbas

AU - Wang, Shanshan

AU - Zhang, Xingchen

AU - Liu, Xiaogang

AU - Xu, Huan

AU - Wang, Wei

AU - Lou, Kaiyan

PY - 2022/3/4

Y1 - 2022/3/4

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AB - A fluorescent probe (COU-LIP-1) for monitoring labile Fe(ii) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(ii)-induced reductive cleavage of the N-O bond results in a turn-on response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(ii) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.

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DO - 10.1039/d1cc06611k

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AN - SCOPUS:85125553814

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VL - 58

SP - 2979

EP - 2982

JO - Chemical Communications

JF - Chemical Communications

IS - 18

ER -

A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis

Abstract

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