A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis

Wanjin Xing; Hang Xu; Huijuan Ma; Syed Ali Abbas Abedi; Shanshan Wang; Xingchen Zhang; Xiaogang Liu; Huan Xu; Wei Wang; Kaiyan Lou

doi:10.1039/d1cc06611k

A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis

Wanjin Xing, Hang Xu, Huijuan Ma, Syed Ali Abbas Abedi, Shanshan Wang, Xingchen Zhang, Xiaogang Liu, Huan Xu, Wei Wang, Kaiyan Lou

Pharmacology and Toxicology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A fluorescent probe (COU-LIP-1) for monitoring labile Fe(ii) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(ii)-induced reductive cleavage of the N-O bond results in a turn-on response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(ii) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.

Original language	English (US)
Pages (from-to)	2979-2982
Number of pages	4
Journal	Chemical Communications
Volume	58
Issue number	18
DOIs	https://doi.org/10.1039/d1cc06611k
State	Published - Mar 4 2022

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/d1cc06611k

Cite this

@article{24077da073364e66b35000230e3e4036,

title = "A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis",

abstract = "A fluorescent probe (COU-LIP-1) for monitoring labile Fe(ii) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(ii)-induced reductive cleavage of the N-O bond results in a turn-on response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(ii) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.",

author = "Wanjin Xing and Hang Xu and Huijuan Ma and Abedi, {Syed Ali Abbas} and Shanshan Wang and Xingchen Zhang and Xiaogang Liu and Huan Xu and Wei Wang and Kaiyan Lou",

note = "Publisher Copyright: This journal is {\textcopyright} The Royal Society of Chemistry",

year = "2022",

month = mar,

day = "4",

doi = "10.1039/d1cc06611k",

language = "English (US)",

volume = "58",

pages = "2979--2982",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "18",

}

TY - JOUR

T1 - A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis

AU - Xing, Wanjin

AU - Xu, Hang

AU - Ma, Huijuan

AU - Abedi, Syed Ali Abbas

AU - Wang, Shanshan

AU - Zhang, Xingchen

AU - Liu, Xiaogang

AU - Xu, Huan

AU - Wang, Wei

AU - Lou, Kaiyan

PY - 2022/3/4

Y1 - 2022/3/4

N2 - A fluorescent probe (COU-LIP-1) for monitoring labile Fe(ii) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(ii)-induced reductive cleavage of the N-O bond results in a turn-on response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(ii) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.

AB - A fluorescent probe (COU-LIP-1) for monitoring labile Fe(ii) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(ii)-induced reductive cleavage of the N-O bond results in a turn-on response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(ii) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.

UR - http://www.scopus.com/inward/record.url?scp=85125553814&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85125553814&partnerID=8YFLogxK

U2 - 10.1039/d1cc06611k

DO - 10.1039/d1cc06611k

M3 - Article

C2 - 35147150

AN - SCOPUS:85125553814

SN - 1359-7345

VL - 58

SP - 2979

EP - 2982

JO - Chemical Communications

JF - Chemical Communications

IS - 18

ER -

A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this