A perspective on the characterization of colloids and macromolecules using asymmetrical flow field-flow fractionation

J. Ray Runyon, Matilda Ulmius, Lars Nilsson

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Asymmetrical flow field-flow fractionation (AF4) is rapidly becoming a technique of choice for the separation and characterization of complex materials. It is capable of fractionating samples over a wide size (~2nm to 50μm in diameter) and molecular weight range (103-1010g/mol). It offers gentle, low shear, low pressure separation conditions which are essential to preserve the structure and aggregation of fragile species. In this paper we illustrate a number of examples where AF4 plays central role in providing detailed and accurate characterization of polydisperse and complex colloidal and macromolecular materials such as proteins, polysaccharides, nanoparticles, and emulsion droplets.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume442
DOIs
StatePublished - Feb 1 2014
Externally publishedYes

Keywords

  • Aggregates
  • Beta-glucans
  • Colloids
  • Emulsions
  • Field-flow fractionation
  • Proteins

ASJC Scopus subject areas

  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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