A perspective on the characterization of colloids and macromolecules using asymmetrical flow field-flow fractionation

J. Ray Runyon; Matilda Ulmius; Lars Nilsson

doi:10.1016/j.colsurfa.2013.04.010

A perspective on the characterization of colloids and macromolecules using asymmetrical flow field-flow fractionation

J. Ray Runyon, Matilda Ulmius, Lars Nilsson

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Asymmetrical flow field-flow fractionation (AF4) is rapidly becoming a technique of choice for the separation and characterization of complex materials. It is capable of fractionating samples over a wide size (~2nm to 50μm in diameter) and molecular weight range (10³-10¹⁰g/mol). It offers gentle, low shear, low pressure separation conditions which are essential to preserve the structure and aggregation of fragile species. In this paper we illustrate a number of examples where AF4 plays central role in providing detailed and accurate characterization of polydisperse and complex colloidal and macromolecular materials such as proteins, polysaccharides, nanoparticles, and emulsion droplets.

Original language	English (US)
Pages (from-to)	25-33
Number of pages	9
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	442
DOIs	https://doi.org/10.1016/j.colsurfa.2013.04.010
State	Published - Feb 1 2014
Externally published	Yes

Keywords

Aggregates
Beta-glucans
Colloids
Emulsions
Field-flow fractionation
Proteins

ASJC Scopus subject areas

Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfa.2013.04.010

Cite this

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abstract = "Asymmetrical flow field-flow fractionation (AF4) is rapidly becoming a technique of choice for the separation and characterization of complex materials. It is capable of fractionating samples over a wide size (~2nm to 50μm in diameter) and molecular weight range (103-1010g/mol). It offers gentle, low shear, low pressure separation conditions which are essential to preserve the structure and aggregation of fragile species. In this paper we illustrate a number of examples where AF4 plays central role in providing detailed and accurate characterization of polydisperse and complex colloidal and macromolecular materials such as proteins, polysaccharides, nanoparticles, and emulsion droplets.",

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AU - Runyon, J. Ray

AU - Ulmius, Matilda

AU - Nilsson, Lars

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AB - Asymmetrical flow field-flow fractionation (AF4) is rapidly becoming a technique of choice for the separation and characterization of complex materials. It is capable of fractionating samples over a wide size (~2nm to 50μm in diameter) and molecular weight range (103-1010g/mol). It offers gentle, low shear, low pressure separation conditions which are essential to preserve the structure and aggregation of fragile species. In this paper we illustrate a number of examples where AF4 plays central role in providing detailed and accurate characterization of polydisperse and complex colloidal and macromolecular materials such as proteins, polysaccharides, nanoparticles, and emulsion droplets.

KW - Aggregates

KW - Beta-glucans

KW - Colloids

KW - Emulsions

KW - Field-flow fractionation

KW - Proteins

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A perspective on the characterization of colloids and macromolecules using asymmetrical flow field-flow fractionation

Abstract

Keywords

ASJC Scopus subject areas

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