A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion

Jie Fan; Xinxin Ding; Yongping Jiang

doi:10.1186/1756-8722-5-73

A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion

Jie Fan, Xinxin Ding, Yongping Jiang

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Stem cell factor (SCF) activates hematopoietic stem cell (HSC) self-renewal and is being used to stimulate the ex vivo expansion of HSCs. The mechanism by which SCF supports expansion of HSCs remains poorly understood. In cord blood ex vivo expansion assays, a newly produced anti-SCF monoclonal antibody (clone 23C8) was found to significantly inhibit the expansion of CD34⁺ cells. This antibody appears to bind directly to a part of SCF that is critical for biological activity toward expansion of CD34⁺ cells, which is located in the first 104 amino acids from the NH₂-terminus.

Original language	English (US)
Article number	73
Journal	Journal of Hematology and Oncology
Volume	5
DOIs	https://doi.org/10.1186/1756-8722-5-73
State	Published - 2012
Externally published	Yes

Keywords

Hematopoietic stem cell
Monoclonal antibody
Stem cell factor
Umbilical cord blood

ASJC Scopus subject areas

Molecular Biology
Hematology
Oncology
Cancer Research

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10.1186/1756-8722-5-73

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title = "A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion",

abstract = "Stem cell factor (SCF) activates hematopoietic stem cell (HSC) self-renewal and is being used to stimulate the ex vivo expansion of HSCs. The mechanism by which SCF supports expansion of HSCs remains poorly understood. In cord blood ex vivo expansion assays, a newly produced anti-SCF monoclonal antibody (clone 23C8) was found to significantly inhibit the expansion of CD34+ cells. This antibody appears to bind directly to a part of SCF that is critical for biological activity toward expansion of CD34+ cells, which is located in the first 104 amino acids from the NH2-terminus.",

keywords = "Hematopoietic stem cell, Monoclonal antibody, Stem cell factor, Umbilical cord blood",

author = "Jie Fan and Xinxin Ding and Yongping Jiang",

note = "Funding Information: This work was supported by State Scientific Key Projects for New Drug Research and Development (2011ZX09102-010-04 and 2011ZX09401-027), China.",

year = "2012",

doi = "10.1186/1756-8722-5-73",

language = "English (US)",

volume = "5",

journal = "Journal of Hematology and Oncology",

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TY - JOUR

T1 - A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion

AU - Fan, Jie

AU - Ding, Xinxin

AU - Jiang, Yongping

N1 - Funding Information: This work was supported by State Scientific Key Projects for New Drug Research and Development (2011ZX09102-010-04 and 2011ZX09401-027), China.

PY - 2012

Y1 - 2012

N2 - Stem cell factor (SCF) activates hematopoietic stem cell (HSC) self-renewal and is being used to stimulate the ex vivo expansion of HSCs. The mechanism by which SCF supports expansion of HSCs remains poorly understood. In cord blood ex vivo expansion assays, a newly produced anti-SCF monoclonal antibody (clone 23C8) was found to significantly inhibit the expansion of CD34+ cells. This antibody appears to bind directly to a part of SCF that is critical for biological activity toward expansion of CD34+ cells, which is located in the first 104 amino acids from the NH2-terminus.

AB - Stem cell factor (SCF) activates hematopoietic stem cell (HSC) self-renewal and is being used to stimulate the ex vivo expansion of HSCs. The mechanism by which SCF supports expansion of HSCs remains poorly understood. In cord blood ex vivo expansion assays, a newly produced anti-SCF monoclonal antibody (clone 23C8) was found to significantly inhibit the expansion of CD34+ cells. This antibody appears to bind directly to a part of SCF that is critical for biological activity toward expansion of CD34+ cells, which is located in the first 104 amino acids from the NH2-terminus.

KW - Hematopoietic stem cell

KW - Monoclonal antibody

KW - Stem cell factor

KW - Umbilical cord blood

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DO - 10.1186/1756-8722-5-73

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SN - 1756-8722

VL - 5

JO - Journal of Hematology and Oncology

JF - Journal of Hematology and Oncology

M1 - 73

ER -

A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion

Abstract

Keywords

ASJC Scopus subject areas

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