@article{13155ab323024fe98ef20bdcdb252a7f,
title = "A Novel, Highly Related Jumbo Family of Bacteriophages That Were Isolated Against Erwinia",
abstract = "Erwinia amylovora is a plant pathogen from the Erwiniaceae family and a causative agent of the devastating agricultural disease fire blight. Here we characterize eight lytic bacteriophages of E. amylovora that we isolated from the Wasatch front (Utah, United States) that are highly similar to vB_EamM_Ea35-70 which was isolated in Ontario, Canada. With the genome size ranging from 271 to 275 kb, this is a novel jumbo family of bacteriophages. These jumbo bacteriophages were further characterized through genomic and proteomic comparison, mass spectrometry, host range and burst size. Their proteomes are highly unstudied, with over 200 putative proteins with no known homologs. The production of 27 of these putative proteins was confirmed by mass spectrometry analysis. These bacteriophages appear to be most similar to bacteriophages that infect Pseudomonas and Ralstonia rather than Enterobacteriales bacteria by protein similarity, however, we were only able to detect infection of Erwinia and the closely related strains of Pantoea.",
keywords = "Agrican357virus, Erwinia, Pantoea, burst size, genome, jumbo bacteriophage, novel, proteome",
author = "Ruchira Sharma and Pielstick, {Brittany A.} and Bell, {Kimberly A.} and Nieman, {Tanner B.} and Stubbs, {Olivia A.} and Yeates, {Edward L.} and Baltrus, {David A.} and Grose, {Julianne H.}",
note = "Funding Information: The authors extend a special thanks to the Science Education Alliance ? Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) for support and training on bacteriophage analysis, Steven Cresawn for training on Phamerator, Majid Ghassemian and BMPMSF at UCSD for carrying out mass spectrometry, George W. Sundin for donating E. amylovora strains, Edward Wilcox from the BYU Sequencing Center, Michael Standing from the BYU Microscopy Lab and Daniel Thompson for helping with phage microscopy, David Parady and Brandon Ekins from Life Science IT at BYU for hosting DNAmaster and PhamDB on BYU server, Tsz Ching Tam for media preparation, Daniel Arens for helping with cloning and Yomesh Sharma for helping with the formatting of the manuscript. The authors also like to thank the following undergraduates from the BYU Phage Hunters? program for their contributions to isolation of the bacteriophages: Garrett Jensen, Jared Kruger, Madison Melville, Trevon Galbraith, Savannah Grossarth, Hannah Ferguson, and Austin Simister. Funding. DB was awarded a grant by the United States Dairy Association. Financial support by The University of Arizona, Brigham Young University, Department of Microbiology and Molecular Biology, and the College of Life Sciences in the form of student wage funds, support, and publication fees is gratefully acknowledged. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2019 Sharma, Pielstick, Bell, Nieman, Stubbs, Yeates, Baltrus and Grose.",
year = "2019",
month = jul,
day = "23",
doi = "10.3389/fmicb.2019.01533",
language = "English (US)",
volume = "10",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",
}