A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein

Satoshi Orita; Akihiko Saiga; Shin Takagi; Tatsuro Tanaka; Koichi Okumura; Yuko Aono; Yorio Hinuma; Hisanaga Igarashi

doi:10.1016/0014-5793(91)81402-T

A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein

Satoshi Orita
, Akihiko Saiga
, Shin Takagi
, Tatsuro Tanaka
, Koichi Okumura
, Yuko Aono
, Yorio Hinuma
, Hisanaga Igarashi

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The pX sequence of human T cell leukemia virus type 1 (HTLV-1) has been thought to be expressed as a doubly spliced mRNA that codes for p40tax, p27rex and p21X. However, we identified a novel alternatively spliced mRNA in the HTLV-1 infected cells by using reverse transcription followed by the polymerase chain reaction. This mRNA contains only the first and third exons of the doubly spliced mRNA and encodes only p21X. Our data that this mRNA is responsible for expressing p21X exists in most of HTLV-1 infected cells strongly suggests that p21X may play a crucial role for HTLV-1 replication.

Original language	English (US)
Pages (from-to)	127-134
Number of pages	8
Journal	FEBS Letters
Volume	295
Issue number	1-3
DOIs	https://doi.org/10.1016/0014-5793(91)81402-T
State	Published - Dec 16 1991
Externally published	Yes

Keywords

Gene product, rex
Human T cell leukemia virus (HTLV-1)
Polymerase chain reaction
PX gene
RNA splicing

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)81402-T

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title = "A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein",

abstract = "The pX sequence of human T cell leukemia virus type 1 (HTLV-1) has been thought to be expressed as a doubly spliced mRNA that codes for p40tax, p27rex and p21X. However, we identified a novel alternatively spliced mRNA in the HTLV-1 infected cells by using reverse transcription followed by the polymerase chain reaction. This mRNA contains only the first and third exons of the doubly spliced mRNA and encodes only p21X. Our data that this mRNA is responsible for expressing p21X exists in most of HTLV-1 infected cells strongly suggests that p21X may play a crucial role for HTLV-1 replication.",

keywords = "Gene product, rex, Human T cell leukemia virus (HTLV-1), Polymerase chain reaction, PX gene, RNA splicing",

author = "Satoshi Orita and Akihiko Saiga and Shin Takagi and Tatsuro Tanaka and Koichi Okumura and Yuko Aono and Yorio Hinuma and Hisanaga Igarashi",

year = "1991",

month = dec,

day = "16",

doi = "10.1016/0014-5793(91)81402-T",

language = "English (US)",

volume = "295",

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journal = "FEBS Letters",

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T1 - A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein

AU - Orita, Satoshi

AU - Saiga, Akihiko

AU - Takagi, Shin

AU - Tanaka, Tatsuro

AU - Okumura, Koichi

AU - Aono, Yuko

AU - Hinuma, Yorio

AU - Igarashi, Hisanaga

PY - 1991/12/16

Y1 - 1991/12/16

N2 - The pX sequence of human T cell leukemia virus type 1 (HTLV-1) has been thought to be expressed as a doubly spliced mRNA that codes for p40tax, p27rex and p21X. However, we identified a novel alternatively spliced mRNA in the HTLV-1 infected cells by using reverse transcription followed by the polymerase chain reaction. This mRNA contains only the first and third exons of the doubly spliced mRNA and encodes only p21X. Our data that this mRNA is responsible for expressing p21X exists in most of HTLV-1 infected cells strongly suggests that p21X may play a crucial role for HTLV-1 replication.

AB - The pX sequence of human T cell leukemia virus type 1 (HTLV-1) has been thought to be expressed as a doubly spliced mRNA that codes for p40tax, p27rex and p21X. However, we identified a novel alternatively spliced mRNA in the HTLV-1 infected cells by using reverse transcription followed by the polymerase chain reaction. This mRNA contains only the first and third exons of the doubly spliced mRNA and encodes only p21X. Our data that this mRNA is responsible for expressing p21X exists in most of HTLV-1 infected cells strongly suggests that p21X may play a crucial role for HTLV-1 replication.

KW - Gene product, rex

KW - Human T cell leukemia virus (HTLV-1)

KW - Polymerase chain reaction

KW - PX gene

KW - RNA splicing

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UR - https://www.scopus.com/pages/publications/0026343682#tab=citedBy

U2 - 10.1016/0014-5793(91)81402-T

DO - 10.1016/0014-5793(91)81402-T

M3 - Article

C2 - 1765142

AN - SCOPUS:0026343682

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VL - 295

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EP - 134

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein

Abstract

Keywords

ASJC Scopus subject areas

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