A novel agent (Endozoicomonas elysicola) responsible for epitheliocystis in cobia Rachycentrum canadum larvae

Mabel Mendoza, Linda Güiza, Ximena Martinez, Xenia Caraballo, Jaime Rojas, Luis Fernando Aranguren, Marcela Salazar

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Aquaculture of cobia has gained popularity in the last decade, and this species is now farmed in several countries in Latin America and Asia. Despite recent improvement in production techniques that allowed the expansion of the industry, little is known about the diseases that affect cobia during the larviculture stage. In this article we investigated the cause of mass mortalities occurring 13-20 d post-hatching in 3 cycles of cobia larviculture. Wet mounts from diseased larvae gills revealed the presence of cyst-like basophilic inclusions. DNA from the cysts was extracted and PCR amplified using the 16S rRNA gene universal primers for prokaryotes. The amplified products were sequenced and analyzed using BLAST, finding a similarity of 99% with Endozoicomonas elysicola, a Gram-negative bacterium. Confirmation of E. elysicola was conducted by designing a specific probe for in situ hybridization. Specific primers were also designed for diagnostic purposes. This is the first report of epitheliocystis in cobia larvae and also the first report of E. elysicola as an epitheliocystis-causing agent.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalDiseases of aquatic organisms
Volume106
Issue number1
DOIs
StatePublished - Sep 24 2013
Externally publishedYes

Keywords

  • Cobia
  • Endozoicomonas elysicola
  • Epitheliocystis
  • Gill inflammation

ASJC Scopus subject areas

  • General Medicine

Fingerprint

Dive into the research topics of 'A novel agent (Endozoicomonas elysicola) responsible for epitheliocystis in cobia Rachycentrum canadum larvae'. Together they form a unique fingerprint.

Cite this