A nonviral minicircle vector for deriving human iPS cells

Fangjun Jia; Kitchener D. Wilson; Ning Sun; Deepak M. Gupta; Mei Huang; Zongjin Li; Nicholas J. Panetta; Zhi Ying Chen; Robert C. Robbins; Mark A. Kay; Michael T. Longaker; Joseph C. Wu

doi:10.1038/nmeth.1426

A nonviral minicircle vector for deriving human iPS cells

Fangjun Jia
, Kitchener D. Wilson
, Ning Sun
, Deepak M. Gupta
, Mei Huang
, Zongjin Li
, Nicholas J. Panetta
, Zhi Ying Chen
, Robert C. Robbins
, Mark A. Kay
, Michael T. Longaker
, Joseph C. Wu

Research output: Contribution to journal › Article › peer-review

621 Scopus citations

Abstract

Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.

Original language	English (US)
Pages (from-to)	197-199
Number of pages	3
Journal	Nature Methods
Volume	7
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.1426
State	Published - Mar 2010
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1426

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@article{c5d55c429ed44ad1a50d930143a01d14,

title = "A nonviral minicircle vector for deriving human iPS cells",

abstract = "Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.",

author = "Fangjun Jia and Wilson, \{Kitchener D.\} and Ning Sun and Gupta, \{Deepak M.\} and Mei Huang and Zongjin Li and Panetta, \{Nicholas J.\} and Chen, \{Zhi Ying\} and Robbins, \{Robert C.\} and Kay, \{Mark A.\} and Longaker, \{Michael T.\} and Wu, \{Joseph C.\}",

note = "Funding Information: We thank A.J. Connolly for assistance with histological analysis, members of the Stanford Functional Genomics Facility and Stanford University PAN Core Facility for assistance with microarrays and A. Cherry for assistance with cytogenetics. We thank funding support from Mallinckrodt Foundation, US National Institutes of Health (NIH) DP2OD004437, HL091453-01A1S109, Burroughs Wellcome Foundation and American Heart Association 0970394N (J.C.W.); NIH R90 DK 07010301, California Institute of Regenerative Medicine T1-00001 and RL1-00662-1, NIH R21 DE018727, RC1HL100490, NIH R21 DE019274, the Oak Foundation and the Hagey Laboratory for Pediatric Regenerative Medicine (M.T.L.); U01HL099776 (R.C.R.).",

year = "2010",

month = mar,

doi = "10.1038/nmeth.1426",

language = "English (US)",

volume = "7",

pages = "197--199",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Research",

number = "3",

}

TY - JOUR

T1 - A nonviral minicircle vector for deriving human iPS cells

AU - Jia, Fangjun

AU - Wilson, Kitchener D.

AU - Sun, Ning

AU - Gupta, Deepak M.

AU - Huang, Mei

AU - Li, Zongjin

AU - Panetta, Nicholas J.

AU - Chen, Zhi Ying

AU - Robbins, Robert C.

AU - Kay, Mark A.

AU - Longaker, Michael T.

AU - Wu, Joseph C.

N1 - Funding Information: We thank A.J. Connolly for assistance with histological analysis, members of the Stanford Functional Genomics Facility and Stanford University PAN Core Facility for assistance with microarrays and A. Cherry for assistance with cytogenetics. We thank funding support from Mallinckrodt Foundation, US National Institutes of Health (NIH) DP2OD004437, HL091453-01A1S109, Burroughs Wellcome Foundation and American Heart Association 0970394N (J.C.W.); NIH R90 DK 07010301, California Institute of Regenerative Medicine T1-00001 and RL1-00662-1, NIH R21 DE018727, RC1HL100490, NIH R21 DE019274, the Oak Foundation and the Hagey Laboratory for Pediatric Regenerative Medicine (M.T.L.); U01HL099776 (R.C.R.).

PY - 2010/3

Y1 - 2010/3

N2 - Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.

AB - Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.

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U2 - 10.1038/nmeth.1426

DO - 10.1038/nmeth.1426

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AN - SCOPUS:77649275936

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SP - 197

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JO - Nature Methods

JF - Nature Methods

IS - 3

ER -

A nonviral minicircle vector for deriving human iPS cells

Abstract

ASJC Scopus subject areas

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