@article{c5d55c429ed44ad1a50d930143a01d14,
title = "A nonviral minicircle vector for deriving human iPS cells",
abstract = "Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.",
author = "Fangjun Jia and Wilson, {Kitchener D.} and Ning Sun and Gupta, {Deepak M.} and Mei Huang and Zongjin Li and Panetta, {Nicholas J.} and Chen, {Zhi Ying} and Robbins, {Robert C.} and Kay, {Mark A.} and Longaker, {Michael T.} and Wu, {Joseph C.}",
note = "Funding Information: We thank A.J. Connolly for assistance with histological analysis, members of the Stanford Functional Genomics Facility and Stanford University PAN Core Facility for assistance with microarrays and A. Cherry for assistance with cytogenetics. We thank funding support from Mallinckrodt Foundation, US National Institutes of Health (NIH) DP2OD004437, HL091453-01A1S109, Burroughs Wellcome Foundation and American Heart Association 0970394N (J.C.W.); NIH R90 DK 07010301, California Institute of Regenerative Medicine T1-00001 and RL1-00662-1, NIH R21 DE018727, RC1HL100490, NIH R21 DE019274, the Oak Foundation and the Hagey Laboratory for Pediatric Regenerative Medicine (M.T.L.); U01HL099776 (R.C.R.).",
year = "2010",
month = mar,
doi = "10.1038/nmeth.1426",
language = "English (US)",
volume = "7",
pages = "197--199",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "3",
}