TY - JOUR
T1 - A new entomopathogenic nematode, Steinernema colombiense n. sp. (Nematoda
T2 - Steinernematidae), from Colombia
AU - López-Núñez, Juan Carlos
AU - Plichta, Kathryn
AU - Góngora-Botero, Carmenza E.
AU - Stock, Patricia S.
N1 - Funding Information:
We are thankful to Y. Flores-Lara for assistance with the scanning electron microscopy images. The authors also express their gratitude to Cenicafé research leaders G. Cadena-Gómez, A.E. Bustillo-P., D. Rodríguez and C. Quintero for their support on the EPN project. This study was funded in part by a Colombia Ministry of the Environment grant (Project ENT1801) and The National Federation of Colombian Coffee Growers, Coffee Research Centre (Cenicafé).
PY - 2008/5/1
Y1 - 2008/5/1
N2 - A new entomopathogenic nematode, Steinernema colombiense n. sp., is described from Colombia. Morphological, molecular (28S and ITS rDNA sequence data) and cross-hybridisation studies were used for diagnostics and identification purposes. In addition, 28S and ITS rDNA sequence data were used to assess evolutionary relationships of the new species with other Steinernema spp. Morphological diagnostic features for S. colombiense n. sp. include morphometric features of the third-stage infective juvenile, including body length of 636 (549-732) μm, narrow body diam. (31 (22-36) μm), position of the excretory pore (35 (31-40) μm), tail length (41 (32-53) μm), D% = 29 (25-33) and E% = 205 (138-284). In addition, males of first and second generations are characterised by the morphology of the spicules and gubernaculum, the number and arrangement of the genital papillae and the excretory pore position (at 67 (56-76) and 54 (46-63) μm, for first and second generations, respectively). In addition to these traits, 28S and ITS rDNA sequences analyses both showed this species to be a distinct and unique entity.
AB - A new entomopathogenic nematode, Steinernema colombiense n. sp., is described from Colombia. Morphological, molecular (28S and ITS rDNA sequence data) and cross-hybridisation studies were used for diagnostics and identification purposes. In addition, 28S and ITS rDNA sequence data were used to assess evolutionary relationships of the new species with other Steinernema spp. Morphological diagnostic features for S. colombiense n. sp. include morphometric features of the third-stage infective juvenile, including body length of 636 (549-732) μm, narrow body diam. (31 (22-36) μm), position of the excretory pore (35 (31-40) μm), tail length (41 (32-53) μm), D% = 29 (25-33) and E% = 205 (138-284). In addition, males of first and second generations are characterised by the morphology of the spicules and gubernaculum, the number and arrangement of the genital papillae and the excretory pore position (at 67 (56-76) and 54 (46-63) μm, for first and second generations, respectively). In addition to these traits, 28S and ITS rDNA sequences analyses both showed this species to be a distinct and unique entity.
KW - Description
KW - Molecular
KW - Morphology
KW - Morphometrics
KW - New species
KW - Phylogeny
KW - South America
KW - Taxonomy
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U2 - 10.1163/156854108784513879
DO - 10.1163/156854108784513879
M3 - Article
AN - SCOPUS:44349194092
SN - 1388-5545
VL - 10
SP - 561
EP - 574
JO - Nematology
JF - Nematology
IS - 4
ER -