A naturally derived, cytocompatible, and architecturally optimized scaffold for tendon and ligament regeneration

Patrick W. Whitlock; Thomas L. Smith; Gary G. Poehling; Jeffrey S. Shilt; Mark Van Dyke

doi:10.1016/j.biomaterials.2007.05.029

A naturally derived, cytocompatible, and architecturally optimized scaffold for tendon and ligament regeneration

Patrick W. Whitlock, Thomas L. Smith, Gary G. Poehling, Jeffrey S. Shilt, Mark Van Dyke

Research output: Contribution to journal › Article › peer-review

112 Scopus citations

Abstract

Tissue-engineered tendon scaffolds have the potential to significantly improve the treatment of tendon and ligament injuries, especially those associated with tumors, trauma, and congenital deficiencies where autograft or allograft tissue might not be available in sufficient quantity for reconstruction. In this study, a tendon scaffold was produced that: (1) has decreased/absent cellular material histologically, as well as significantly decreased DNA content in comparison with the material it is derived from-fresh-frozen flexor digitorum profundus tendon; (2) is cytocompatible in vitro; (3) has been modified to produce increased pore size and porosity; (4) retains 76-78% of the tensile properties of the material it is derived from; (5) is readily infiltrated by fibroblast-like, mononuclear host cells; and (6) does not exhibit a host-cell-mediated foreign-body immune response after implantation in vivo.

Original language	English (US)
Pages (from-to)	4321-4329
Number of pages	9
Journal	Biomaterials
Volume	28
Issue number	29
DOIs	https://doi.org/10.1016/j.biomaterials.2007.05.029
State	Published - Oct 2007
Externally published	Yes

Keywords

Ligament
Porosity
Scaffold
Tendon

ASJC Scopus subject areas

Mechanics of Materials
Ceramics and Composites
Bioengineering
Biophysics
Biomaterials

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10.1016/j.biomaterials.2007.05.029

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title = "A naturally derived, cytocompatible, and architecturally optimized scaffold for tendon and ligament regeneration",

abstract = "Tissue-engineered tendon scaffolds have the potential to significantly improve the treatment of tendon and ligament injuries, especially those associated with tumors, trauma, and congenital deficiencies where autograft or allograft tissue might not be available in sufficient quantity for reconstruction. In this study, a tendon scaffold was produced that: (1) has decreased/absent cellular material histologically, as well as significantly decreased DNA content in comparison with the material it is derived from-fresh-frozen flexor digitorum profundus tendon; (2) is cytocompatible in vitro; (3) has been modified to produce increased pore size and porosity; (4) retains 76-78% of the tensile properties of the material it is derived from; (5) is readily infiltrated by fibroblast-like, mononuclear host cells; and (6) does not exhibit a host-cell-mediated foreign-body immune response after implantation in vivo.",

keywords = "Ligament, Porosity, Scaffold, Tendon",

author = "Whitlock, {Patrick W.} and Smith, {Thomas L.} and Poehling, {Gary G.} and Shilt, {Jeffrey S.} and {Van Dyke}, Mark",

note = "Funding Information: The authors thank Rob Poindexter (Wayne Farms) and the Musculoskeletal Transplant Foundation (MTF{\texttrademark}) for providing tissue, Cathy Mathis for technical assistance, and Saami Yazdani, Kathryne Stabile, and Daniel Eberli for helpful discussions. Patrick Whitlock would like to gratefully acknowledge funding support from the Arthroscopy Association of North America, Smith and Nephew, and the Orthopaedic Research and Education Foundation. The authors also gratefully acknowledge partial funding support provided by the Wake Forest Institute for Regenerative Medicine and the Department of Orthopaedic Surgery.",

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AU - Van Dyke, Mark

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A naturally derived, cytocompatible, and architecturally optimized scaffold for tendon and ligament regeneration

Abstract

Keywords

ASJC Scopus subject areas

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