A molecular mechanism for the procentriole recruitment of Ana2

Tiffany A. McLamarrah, Sarah K. Speed, John M. Ryniawec, Daniel W. Buster, Carey J. Fagerstrom, Brian J. Galletta, Nasser M. Rusan, Gregory C. Rogers

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


During centriole duplication, a preprocentriole forms at a single site on the mother centriole through a process that includes the hierarchical recruitment of a conserved set of proteins, including the Polo-like kinase 4 (Plk4), Ana2/STIL, and the cartwheel protein Sas6. Ana2/STIL is critical for procentriole assembly, and its recruitment is controlled by the kinase activity of Plk4, but how this works remains poorly understood. A structural motif called the G-box in the centriole outer wall protein Sas4 interacts with a short region in the N terminus of Ana2/STIL. Here, we show that binding of Ana2 to the Sas4 G-box enables hyperphosphorylation of the Ana2 N terminus by Plk4. Hyperphosphorylation increases the affinity of the Ana2-G-box interaction, and, consequently, promotes the accumulation of Ana2 at the procentriole to induce daughter centriole formation.

Original languageEnglish (US)
JournalJournal of Cell Biology
Issue number2
StatePublished - Feb 3 2020

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'A molecular mechanism for the procentriole recruitment of Ana2'. Together they form a unique fingerprint.

Cite this