A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome

A. Kleinhofs; A. Kilian; M. A. Saghai Maroof; R. M. Biyashev; P. Hayes; F. Q. Chen; N. Lapitan; A. Fenwick; T. K. Blake; V. Kanazin; E. Ananiev; L. Dahleen; D. Kudrna; J. Bollinger; S. J. Knapp; B. Liu; M. Sorrells; M. Heun; J. D. Franckowiak; D. Hoffman; R. Skadsen; B. J. Steffenson

doi:10.1007/BF00222660

A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome

A. Kleinhofs, A. Kilian, M. A. Saghai Maroof, R. M. Biyashev, P. Hayes, F. Q. Chen, N. Lapitan, A. Fenwick, T. K. Blake, V. Kanazin, E. Ananiev, L. Dahleen, D. Kudrna, J. Bollinger, S. J. Knapp, B. Liu, M. Sorrells, M. Heun, J. D. Franckowiak, D. HoffmanR. Skadsen, B. J. Steffenson

Research output: Contribution to journal › Article › peer-review

605 Scopus citations

Abstract

A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.

Original language	English (US)
Pages (from-to)	705-712
Number of pages	8
Journal	Theoretical and Applied Genetics
Volume	86
Issue number	6
DOIs	https://doi.org/10.1007/BF00222660
State	Published - Jul 1993
Externally published	Yes

Keywords

Barley
Centromeres
Genome
Mapping
RFLP

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Genetics

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10.1007/BF00222660

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Kleinhofs, A., Kilian, A., Saghai Maroof, M. A., Biyashev, R. M., Hayes, P., Chen, F. Q., Lapitan, N., Fenwick, A., Blake, T. K., Kanazin, V., Ananiev, E., Dahleen, L., Kudrna, D., Bollinger, J., Knapp, S. J., Liu, B., Sorrells, M., Heun, M., Franckowiak, J. D., ... Steffenson, B. J. (1993). A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theoretical and Applied Genetics, 86(6), 705-712. https://doi.org/10.1007/BF00222660

Kleinhofs, A, Kilian, A, Saghai Maroof, MA, Biyashev, RM, Hayes, P, Chen, FQ, Lapitan, N, Fenwick, A, Blake, TK, Kanazin, V, Ananiev, E, Dahleen, L, Kudrna, D, Bollinger, J, Knapp, SJ, Liu, B, Sorrells, M, Heun, M, Franckowiak, JD, Hoffman, D, Skadsen, R & Steffenson, BJ 1993, 'A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome', Theoretical and Applied Genetics, vol. 86, no. 6, pp. 705-712. https://doi.org/10.1007/BF00222660

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abstract = "A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.",

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AU - Kilian, A.

AU - Saghai Maroof, M. A.

AU - Biyashev, R. M.

AU - Hayes, P.

AU - Chen, F. Q.

AU - Lapitan, N.

AU - Fenwick, A.

AU - Blake, T. K.

AU - Kanazin, V.

AU - Ananiev, E.

AU - Dahleen, L.

AU - Kudrna, D.

AU - Bollinger, J.

AU - Knapp, S. J.

AU - Liu, B.

AU - Sorrells, M.

AU - Heun, M.

AU - Franckowiak, J. D.

AU - Hoffman, D.

AU - Skadsen, R.

AU - Steffenson, B. J.

PY - 1993/7

Y1 - 1993/7

N2 - A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.

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KW - Centromeres

KW - Genome

KW - Mapping

KW - RFLP

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A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome

Abstract

Keywords

ASJC Scopus subject areas

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