A modified assay for blood coagulation factor X111 using enzyme immobilisation on Staphylococcus aureus: preliminary evaluation

T. C. Whyard; R. J. Ablin

doi:10.1016/0268-9499(91)90055-9

A modified assay for blood coagulation factor X111 using enzyme immobilisation on Staphylococcus aureus: preliminary evaluation

T. C. Whyard, R. J. Ablin

Research output: Contribution to journal › Article › peer-review

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Abstract

The binding of anti-factor (F) XIII antibody to protein A on Staphylococcus aureus (Staph Aureus) was utilised to selectively remove FXIII (a protransglutaminase) from the sample. Following binding by specific antibody, the presence of the transglutaminase was then evaluated by a filter paper assay. Used in conjunction with the filter paper assay, preliminary evaluation of the Staph Aureus assay appears to provide increased sensitivity for quantification of FXIII activity in fibrinogen containing samples over that of the filter paper assay alone. In addition, the modified assay circumvents the requirement to remove or neutralise endogenous fibrinogen (in applicable samples).

Original language	English (US)
Pages (from-to)	127-130
Number of pages	4
Journal	Fibrinolysis and Proteolysis
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/0268-9499(91)90055-9
State	Published - Apr 1991
Externally published	Yes

Keywords

Enzyme immobilisation
Factor XIII
Protein A (Staphylococcus aureus)
Transglutaminase

ASJC Scopus subject areas

Hematology

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10.1016/0268-9499(91)90055-9

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title = "A modified assay for blood coagulation factor X111 using enzyme immobilisation on Staphylococcus aureus: preliminary evaluation",

abstract = "The binding of anti-factor (F) XIII antibody to protein A on Staphylococcus aureus (Staph Aureus) was utilised to selectively remove FXIII (a protransglutaminase) from the sample. Following binding by specific antibody, the presence of the transglutaminase was then evaluated by a filter paper assay. Used in conjunction with the filter paper assay, preliminary evaluation of the Staph Aureus assay appears to provide increased sensitivity for quantification of FXIII activity in fibrinogen containing samples over that of the filter paper assay alone. In addition, the modified assay circumvents the requirement to remove or neutralise endogenous fibrinogen (in applicable samples).",

keywords = "Enzyme immobilisation, Factor XIII, Protein A (Staphylococcus aureus), Transglutaminase",

author = "Whyard, {T. C.} and Ablin, {R. J.}",

year = "1991",

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T1 - A modified assay for blood coagulation factor X111 using enzyme immobilisation on Staphylococcus aureus

T2 - preliminary evaluation

AU - Whyard, T. C.

AU - Ablin, R. J.

PY - 1991/4

Y1 - 1991/4

N2 - The binding of anti-factor (F) XIII antibody to protein A on Staphylococcus aureus (Staph Aureus) was utilised to selectively remove FXIII (a protransglutaminase) from the sample. Following binding by specific antibody, the presence of the transglutaminase was then evaluated by a filter paper assay. Used in conjunction with the filter paper assay, preliminary evaluation of the Staph Aureus assay appears to provide increased sensitivity for quantification of FXIII activity in fibrinogen containing samples over that of the filter paper assay alone. In addition, the modified assay circumvents the requirement to remove or neutralise endogenous fibrinogen (in applicable samples).

AB - The binding of anti-factor (F) XIII antibody to protein A on Staphylococcus aureus (Staph Aureus) was utilised to selectively remove FXIII (a protransglutaminase) from the sample. Following binding by specific antibody, the presence of the transglutaminase was then evaluated by a filter paper assay. Used in conjunction with the filter paper assay, preliminary evaluation of the Staph Aureus assay appears to provide increased sensitivity for quantification of FXIII activity in fibrinogen containing samples over that of the filter paper assay alone. In addition, the modified assay circumvents the requirement to remove or neutralise endogenous fibrinogen (in applicable samples).

KW - Enzyme immobilisation

KW - Factor XIII

KW - Protein A (Staphylococcus aureus)

KW - Transglutaminase

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JF - Fibrinolysis and Proteolysis

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ER -

A modified assay for blood coagulation factor X111 using enzyme immobilisation on Staphylococcus aureus: preliminary evaluation

Abstract

Keywords

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