The binding of anti-factor (F) XIII antibody to protein A on Staphylococcus aureus (Staph Aureus) was utilised to selectively remove FXIII (a protransglutaminase) from the sample. Following binding by specific antibody, the presence of the transglutaminase was then evaluated by a filter paper assay. Used in conjunction with the filter paper assay, preliminary evaluation of the Staph Aureus assay appears to provide increased sensitivity for quantification of FXIII activity in fibrinogen containing samples over that of the filter paper assay alone. In addition, the modified assay circumvents the requirement to remove or neutralise endogenous fibrinogen (in applicable samples).
- Enzyme immobilisation
- Factor XIII
- Protein A (Staphylococcus aureus)
ASJC Scopus subject areas