A method to detect enteroviruses in sewage sludge-amended soil using the PCR

T. M. Straub; I. L. Pepper; M. Abbaszadegan; C. P. Gerba

doi:10.1128/aem.60.3.1014-1017.1994

A method to detect enteroviruses in sewage sludge-amended soil using the PCR

T. M. Straub, I. L. Pepper, M. Abbaszadegan, C. P. Gerba

Research output: Contribution to journal › Comment/debate › peer-review

35 Scopus citations

Abstract

PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.

Original language	English (US)
Pages (from-to)	1014-1017
Number of pages	4
Journal	Applied and environmental microbiology
Volume	60
Issue number	3
DOIs	https://doi.org/10.1128/aem.60.3.1014-1017.1994
State	Published - 1994

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/aem.60.3.1014-1017.1994

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title = "A method to detect enteroviruses in sewage sludge-amended soil using the PCR",

abstract = "PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.",

author = "Straub, {T. M.} and Pepper, {I. L.} and M. Abbaszadegan and Gerba, {C. P.}",

year = "1994",

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AU - Pepper, I. L.

AU - Abbaszadegan, M.

AU - Gerba, C. P.

PY - 1994

Y1 - 1994

N2 - PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.

AB - PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.

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U2 - 10.1128/aem.60.3.1014-1017.1994

DO - 10.1128/aem.60.3.1014-1017.1994

M3 - Comment/debate

C2 - 8161168

AN - SCOPUS:0028348264

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JO - Applied and environmental microbiology

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ER -

A method to detect enteroviruses in sewage sludge-amended soil using the PCR

Abstract

ASJC Scopus subject areas

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