TY - JOUR
T1 - A method for determining ascaris viability based on early-to-late stage in-vitro ova development
AU - Schmitz, Bradley W.
AU - Pearce-Walker, Jennifer
AU - Gerba, Charles P.
AU - Pepper, Ian L.
N1 - Funding Information:
and technical assistance. We would like to thank an anonymous wastewater treatment facility in southern arizona for supplying biosolids and materials. this study was supported by the national Science foundation (nSf) Water and environmental technology (Wet) center, the University of arizona. We would also like to acknowledge the nSf-Wet center for providing support to Bradley Schmitz and Jennifer Pearce-Walker during this project.
Funding Information:
The authors would like to thank Ms. Maria Campillo and Ms. Emily Wall for their laboratory contributionand technical assistance. We would like to thank an anonymous wastewater treatment facility in southern Arizona for supplying biosolids and materials. This study was supported by the National Science Foundation (NSF) Water and Environmental Technology (WET) Center, The University of Arizona. We would also like to acknowledge the NSF-WET Center for providing support to Bradley Schmitz and Jennifer Pearce-Walker during this project.
Publisher Copyright:
© 2016 DEStech Publications, Inc.
PY - 2016
Y1 - 2016
N2 - This study suggests a new method for determining the viability of Ascaris spp. ova, based on in-vitro early-to-late stage development of ova. This method includes stages prior to larval development, providing an estimation of potential viability. After application of biosolids onto soil and exposure to 7°C, 22°C, or 37°C for 45 days, ova were microscopically distinguished as viable or non-viable according to progression through development categories. Results were compared to viability estimates from current methods that distinguish viable ova as motile larva. Results suggest conventional techniques underestimate viability, whereas the new method provides a more conservative approach.
AB - This study suggests a new method for determining the viability of Ascaris spp. ova, based on in-vitro early-to-late stage development of ova. This method includes stages prior to larval development, providing an estimation of potential viability. After application of biosolids onto soil and exposure to 7°C, 22°C, or 37°C for 45 days, ova were microscopically distinguished as viable or non-viable according to progression through development categories. Results were compared to viability estimates from current methods that distinguish viable ova as motile larva. Results suggest conventional techniques underestimate viability, whereas the new method provides a more conservative approach.
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U2 - 10.12783/issn.1544-8053/13/4/5
DO - 10.12783/issn.1544-8053/13/4/5
M3 - Article
AN - SCOPUS:84994602818
SN - 1544-8053
VL - 13
SP - 275
EP - 286
JO - Journal of Residuals Science and Technology
JF - Journal of Residuals Science and Technology
IS - 4
ER -