TY - JOUR
T1 - A member of a new repeated sequence family which is conserved throughout eucaryotic evolution is found between the human δ and β globin genes
AU - Miesfeld, Roger
AU - Krystal, Mark
AU - Amheim, Norman
N1 - Funding Information:
ACKNOWLEDGEMENTS We wish to thank Sally Spence and Dr.'s Arthur Bank, Prescott Deininger, Jerry Slightom and Jim Broach for their gifts of DNA samples and Dr. Oliver Smithies for providing us with a copy of a manuscript prior to its publication. We thank Dee Sedivec for preparation of the manuscript. We also acknowledge the NIH supported SUMEX-AIM computer facility at Stanford University. This research was supported by a grant from the NIGMS.
PY - 1981/11/25
Y1 - 1981/11/25
N2 - A new class of human interspersed repeated sequences distinct from the Alul family was found by screening a human gene library with a mouse ribosomal gene non-transcribed spacer probe (rDNA NTS). A member of this sequence family was localized to a 251 bp segment between the human δ and β globin genes: a region previously judged to be devoid of repeated DNA. The complete nucleotide sequence of this segment revealed a tandem block of 17 TG dinucleotides, a feature hypothesized by others to be a recombination hot spot responsible for gene conversion in the γ globin locus region. When the genomes of Xenopus, pigeon, slime mold and yeast were examined, reiterated sequences homologous to both the mouse rDNA NTS and human globin repeat were found in every case. The discovery of this extraordinarily conserved repeated sequence family appears to have depended upon not using salmon sperm DNA during hybridization. The use of eucaryotic carrier DNA may bias the search for repeated sequences against any which may be highly conserved during eucaryotic evolution.
AB - A new class of human interspersed repeated sequences distinct from the Alul family was found by screening a human gene library with a mouse ribosomal gene non-transcribed spacer probe (rDNA NTS). A member of this sequence family was localized to a 251 bp segment between the human δ and β globin genes: a region previously judged to be devoid of repeated DNA. The complete nucleotide sequence of this segment revealed a tandem block of 17 TG dinucleotides, a feature hypothesized by others to be a recombination hot spot responsible for gene conversion in the γ globin locus region. When the genomes of Xenopus, pigeon, slime mold and yeast were examined, reiterated sequences homologous to both the mouse rDNA NTS and human globin repeat were found in every case. The discovery of this extraordinarily conserved repeated sequence family appears to have depended upon not using salmon sperm DNA during hybridization. The use of eucaryotic carrier DNA may bias the search for repeated sequences against any which may be highly conserved during eucaryotic evolution.
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U2 - 10.1093/nar/9.22.5931
DO - 10.1093/nar/9.22.5931
M3 - Article
C2 - 6273813
AN - SCOPUS:0019771736
SN - 0305-1048
VL - 9
SP - 5931
EP - 5948
JO - Nucleic acids research
JF - Nucleic acids research
IS - 22
ER -