A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division

G. C. Rogers; K. K. Chui; E. W. Lee; K. P. Wedaman; D. J. Sharp; G. Holland; R. L. Morris; J. M. Scholey

doi:10.1083/jcb.150.3.499

A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division

G. C. Rogers, K. K. Chui, E. W. Lee, K. P. Wedaman, D. J. Sharp, G. Holland, R. L. Morris, J. M. Scholey

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

Original language	English (US)
Pages (from-to)	499-511
Number of pages	13
Journal	Journal of Cell Biology
Volume	150
Issue number	3
DOIs	https://doi.org/10.1083/jcb.150.3.499
State	Published - Aug 7 2000

Keywords

Kinesin
Mitosis
Sea urchin
Xklp2
Y-tubulin

ASJC Scopus subject areas

Cell Biology

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10.1083/jcb.150.3.499

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title = "A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division",

abstract = "We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.",

keywords = "Kinesin, Mitosis, Sea urchin, Xklp2, Y-tubulin",

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AU - Rogers, G. C.

AU - Chui, K. K.

AU - Lee, E. W.

AU - Wedaman, K. P.

AU - Sharp, D. J.

AU - Holland, G.

AU - Morris, R. L.

AU - Scholey, J. M.

PY - 2000/8/7

Y1 - 2000/8/7

N2 - We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

AB - We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

KW - Kinesin

KW - Mitosis

KW - Sea urchin

KW - Xklp2

KW - Y-tubulin

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A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division

Abstract

Keywords

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