A human hair keratin hydrogel scaffold enhances median nerve regeneration in nonhuman primates: An electrophysiological and histological study

Lauren A. Pace, Johannes F. Plate, Sandeep Mannava, Jonathan C. Barnwell, L. Andrew Koman, Zhongyu Li, Thomas L. Smith, Mark Van Dyke

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

A human hair keratin biomaterial hydrogel scaffold was evaluated as a nerve conduit luminal filler following median nerve transection injury in 10 Macaca fascicularis nonhuman primates (NHP). A 1 cm nerve gap was grafted with a NeuraGen® collagen conduit filled with either saline or keratin hydrogel and nerve regeneration was evaluated by electrophysiology for a period of 12 months. The keratin hydrogel-grafted nerves showed significant improvement in return of compound motor action potential (CMAP) latency and recovery of baseline nerve conduction velocity (NCV) compared with the saline-treated nerves. Histological evaluation was performed on retrieved median nerves and abductor pollicis brevis (APB) muscles at 12 months. Nerve histomorphometry showed a significantly larger nerve area in the keratin group compared with the saline group and the keratin APB muscles had a significantly higher myofiber density than the saline group. This is the first published study to show that an acellular biomaterial hydrogel conduit filler can be used to enhance peripheral nerve regeneration and motor recovery in an NHP model.

Original languageEnglish (US)
Pages (from-to)507-517
Number of pages11
JournalTissue Engineering - Part A
Volume20
Issue number3-4
DOIs
StatePublished - Feb 1 2014
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

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