TY - GEN
T1 - A high-performance microsystem for isolating viable circulating tumor cells
AU - Zheng, X. J.
AU - Cheung, L. S.L.
AU - Schroeder, J. A.
AU - Jiang, L.
AU - Zohar, Y.
PY - 2011
Y1 - 2011
N2 - The attachment and detachment of target cancer cells from homogeneous and binary mixtures in antibody-functionalized microchannels have been studied experimentally. Under the same intermediate flow rate, the attachment rate was found to be higher, and detachment flow rate was lower, for cell lines expressing the target receptor at a higher level. For cells that do not express the target receptor, the attachment rate was much lower but did not diminish, due to non-specific binding, and the detachment rate was much higher. The bio-functional microfluidic system performance in selectively isolating target cells from binary mixtures is quantitatively characterized. While the system sensitivity is typically very high, almost 100%, the specificity is lower than 90%. Applying a unique flow scheme of a slow flow rate, for maximum capture of target cells, followed by a faster flow rate, for maximum removal of non-target cells, the specificity is enhanced to levels above 95%, even for mixtures with target cells present at 11,000 relative concentration ratio.
AB - The attachment and detachment of target cancer cells from homogeneous and binary mixtures in antibody-functionalized microchannels have been studied experimentally. Under the same intermediate flow rate, the attachment rate was found to be higher, and detachment flow rate was lower, for cell lines expressing the target receptor at a higher level. For cells that do not express the target receptor, the attachment rate was much lower but did not diminish, due to non-specific binding, and the detachment rate was much higher. The bio-functional microfluidic system performance in selectively isolating target cells from binary mixtures is quantitatively characterized. While the system sensitivity is typically very high, almost 100%, the specificity is lower than 90%. Applying a unique flow scheme of a slow flow rate, for maximum capture of target cells, followed by a faster flow rate, for maximum removal of non-target cells, the specificity is enhanced to levels above 95%, even for mixtures with target cells present at 11,000 relative concentration ratio.
KW - Circulating tumor cells
KW - Microchannels
KW - Sensitivity
KW - Specificity
KW - cell isolation
UR - http://www.scopus.com/inward/record.url?scp=80052120269&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052120269&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2011.5969382
DO - 10.1109/TRANSDUCERS.2011.5969382
M3 - Conference contribution
AN - SCOPUS:80052120269
SN - 9781457701573
T3 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
SP - 226
EP - 229
BT - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
T2 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Y2 - 5 June 2011 through 9 June 2011
ER -