A gradient-free method for the purification of infective dengue virus for protein-level investigations

Stephanie M. Jensen; Celina T. Nguyen; John C. Jewett

doi:10.1016/j.jviromet.2016.05.017

A gradient-free method for the purification of infective dengue virus for protein-level investigations

Stephanie M. Jensen, Celina T. Nguyen, John C. Jewett

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Dengue virus (DENV) is a mosquito-transmitted flavivirus that infects approximately 100 million people annually. Multi-day protocols for purification of DENV reduce the infective titer due to viral sensitivity to both temperature and pH. Herein we describe a 5-h protocol for the purification of all DENV serotypes, utilizing traditional gradient-free ultracentrifugation followed by selective virion precipitation. This protocol allows for the separation of DENV from contaminating proteins - including intact C6/36 densovirus, for the production of infective virus at high concentration for protein-level analysis.

Original language	English (US)
Pages (from-to)	125-130
Number of pages	6
Journal	Journal of Virological Methods
Volume	235
DOIs	https://doi.org/10.1016/j.jviromet.2016.05.017
State	Published - Sep 1 2016

Keywords

Aedes albopictus
Dengue virus
Densovirus
Infectivity
Precipitation
Purification

ASJC Scopus subject areas

Virology

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10.1016/j.jviromet.2016.05.017

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title = "A gradient-free method for the purification of infective dengue virus for protein-level investigations",

abstract = "Dengue virus (DENV) is a mosquito-transmitted flavivirus that infects approximately 100 million people annually. Multi-day protocols for purification of DENV reduce the infective titer due to viral sensitivity to both temperature and pH. Herein we describe a 5-h protocol for the purification of all DENV serotypes, utilizing traditional gradient-free ultracentrifugation followed by selective virion precipitation. This protocol allows for the separation of DENV from contaminating proteins - including intact C6/36 densovirus, for the production of infective virus at high concentration for protein-level analysis.",

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AU - Jensen, Stephanie M.

AU - Nguyen, Celina T.

AU - Jewett, John C.

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AB - Dengue virus (DENV) is a mosquito-transmitted flavivirus that infects approximately 100 million people annually. Multi-day protocols for purification of DENV reduce the infective titer due to viral sensitivity to both temperature and pH. Herein we describe a 5-h protocol for the purification of all DENV serotypes, utilizing traditional gradient-free ultracentrifugation followed by selective virion precipitation. This protocol allows for the separation of DENV from contaminating proteins - including intact C6/36 densovirus, for the production of infective virus at high concentration for protein-level analysis.

KW - Aedes albopictus

KW - Dengue virus

KW - Densovirus

KW - Infectivity

KW - Precipitation

KW - Purification

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

ER -

A gradient-free method for the purification of infective dengue virus for protein-level investigations

Abstract

Keywords

ASJC Scopus subject areas

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