TY - JOUR
T1 - A general procedure for evaluation of immunological relevance of synthetic peptides
T2 - Peptides synthesized on paper in enzyme-linked immunosorbent assay
AU - Krchňák, Viktor
AU - Vágner, Josef
AU - Novák, Jan
AU - Suchánková, Alena
AU - Roubal, Jaroslav
PY - 1990/8/15
Y1 - 1990/8/15
N2 - Multiple continuous-flow solid-phase peptide synthesis has been adapted for synthesis of peptides on a cellulose carrier (Whatman 3MM paper). Paper-bound synthetic peptides that represent antigenic determinants of particular proteins detected antibodies against the respective proteins in an enzyme-linked immunosorbent assay. The method is applied to the synthesis, and use in site-directed serology, of four peptides derived from the gp41 glycoprotein of HIV, the Epstein-Barr virus-determined nuclear antigen-1 and VCA proteins of the Epstein-Barr virus, and the early region of human papillomavirus type 11.
AB - Multiple continuous-flow solid-phase peptide synthesis has been adapted for synthesis of peptides on a cellulose carrier (Whatman 3MM paper). Paper-bound synthetic peptides that represent antigenic determinants of particular proteins detected antibodies against the respective proteins in an enzyme-linked immunosorbent assay. The method is applied to the synthesis, and use in site-directed serology, of four peptides derived from the gp41 glycoprotein of HIV, the Epstein-Barr virus-determined nuclear antigen-1 and VCA proteins of the Epstein-Barr virus, and the early region of human papillomavirus type 11.
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U2 - 10.1016/0003-2697(90)90047-D
DO - 10.1016/0003-2697(90)90047-D
M3 - Article
C2 - 1703730
AN - SCOPUS:0025121476
SN - 0003-2697
VL - 189
SP - 80
EP - 83
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -