A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate

J. Antoinette Killian; Theodore P. Trouard; Denise V. Greathouse; Vladimir Chupin; Göran Lindblom

doi:10.1016/0014-5793(94)00594-X

A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate

J. Antoinette Killian, Theodore P. Trouard, Denise V. Greathouse, Vladimir Chupin, Göran Lindblom

Biomedical Engineering

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49 Scopus citations

Abstract

A new method is reported for the incorporation of hydrophobic peptides into sodium dodecyl sulphate (SDS) micelles. First, a homogeneous solution of peptide and detergent is obtained by adding the peptide in trifluoroethanol to an equal volume of an aqueous solution of SDS. Upon subsequent addition of excess water, mixed peptide-SDS micelles are formed. Next, all solvent is removed by lyophilization and an appropriate amount of water is added to the dry powder. For various hydrophobic peptides this was shown to yield clear and stable solutions that are highly concentrated and suitable for characterization by spectroscopic techniques.

Original language	English (US)
Pages (from-to)	161-165
Number of pages	5
Journal	FEBS Letters
Volume	348
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(94)00594-X
State	Published - Jul 11 1994

Keywords

Circular dichroism
Gramicidin
Micelle
Peptide structure
Signal peptide
Two-dimensional NMR

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(94)00594-X

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title = "A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate",

abstract = "A new method is reported for the incorporation of hydrophobic peptides into sodium dodecyl sulphate (SDS) micelles. First, a homogeneous solution of peptide and detergent is obtained by adding the peptide in trifluoroethanol to an equal volume of an aqueous solution of SDS. Upon subsequent addition of excess water, mixed peptide-SDS micelles are formed. Next, all solvent is removed by lyophilization and an appropriate amount of water is added to the dry powder. For various hydrophobic peptides this was shown to yield clear and stable solutions that are highly concentrated and suitable for characterization by spectroscopic techniques.",

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T1 - A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate

AU - Killian, J. Antoinette

AU - Trouard, Theodore P.

AU - Greathouse, Denise V.

AU - Chupin, Vladimir

AU - Lindblom, Göran

PY - 1994/7/11

Y1 - 1994/7/11

N2 - A new method is reported for the incorporation of hydrophobic peptides into sodium dodecyl sulphate (SDS) micelles. First, a homogeneous solution of peptide and detergent is obtained by adding the peptide in trifluoroethanol to an equal volume of an aqueous solution of SDS. Upon subsequent addition of excess water, mixed peptide-SDS micelles are formed. Next, all solvent is removed by lyophilization and an appropriate amount of water is added to the dry powder. For various hydrophobic peptides this was shown to yield clear and stable solutions that are highly concentrated and suitable for characterization by spectroscopic techniques.

AB - A new method is reported for the incorporation of hydrophobic peptides into sodium dodecyl sulphate (SDS) micelles. First, a homogeneous solution of peptide and detergent is obtained by adding the peptide in trifluoroethanol to an equal volume of an aqueous solution of SDS. Upon subsequent addition of excess water, mixed peptide-SDS micelles are formed. Next, all solvent is removed by lyophilization and an appropriate amount of water is added to the dry powder. For various hydrophobic peptides this was shown to yield clear and stable solutions that are highly concentrated and suitable for characterization by spectroscopic techniques.

KW - Circular dichroism

KW - Gramicidin

KW - Micelle

KW - Peptide structure

KW - Signal peptide

KW - Two-dimensional NMR

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JO - FEBS Letters

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A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate

Abstract

Keywords

ASJC Scopus subject areas

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