A Free Radical Scavenger (Lazaroid U75412E) Attenuates Tumor Necrosis Factor-Alpha Generation in a Rabbit Model of Smoke-Induced Lung Injury

Shengjun Wang; R. Clark Lantz; Evelyn D. Rider; Guan Jie Chen; Veronica Breceda; Allison M. Hays; Raymond F. Robledo; Brian J. Tollinger; S. V.R. Dinesh; Mark L. Witten

doi:10.1159/000196704

A Free Radical Scavenger (Lazaroid U75412E) Attenuates Tumor Necrosis Factor-Alpha Generation in a Rabbit Model of Smoke-Induced Lung Injury

Shengjun Wang
, R. Clark Lantz
, Evelyn D. Rider
, Guan Jie Chen
, Veronica Breceda
, Allison M. Hays
, Raymond F. Robledo
, Brian J. Tollinger
, S. V.R. Dinesh
, Mark L. Witten

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The lazaroid (21-aminosteroid) analogue U75412E was evaluated in rabbits exposed to diesel fuel-polycarbonate plastic smoke. Inhalation of a total of 4.6 mg U75412E aerosolized at a rate of 1.53 mg/min for 3 min before or after smoke significantly prevented or limited the extent of alveolar hypoventilation, interstitial edema, and tumor necrosis factor-α (TNF-α) by pulmonary alveolar macrophages (PAM) ex vivo observed at 2 h. The smoke-induced changes in wet lung/body weight ratios and the production of superoxide (O₂⁻ by PAM ex vivo were also attenuated by the drug treatment after smoke exposure (p ¼ 0.05). This study suggests that lazaroids may ameliorate the oxygen-radical-initiated cytokine processes and inflammation cascade as a result of the smoke insult.

Original language	English (US)
Pages (from-to)	358-363
Number of pages	6
Journal	Respiration
Volume	64
Issue number	5
DOIs	https://doi.org/10.1159/000196704
State	Published - Jan 1 1997
Externally published	Yes

Keywords

Acute lung injury
Lazaroid
Pulmonary alveolar macrophages
Smoke
Superoxide
Tumor necrosis factor-andα

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine

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10.1159/000196704

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@article{efcbd5962d9143aaab0d879c6ed11022,

title = "A Free Radical Scavenger (Lazaroid U75412E) Attenuates Tumor Necrosis Factor-Alpha Generation in a Rabbit Model of Smoke-Induced Lung Injury",

abstract = "The lazaroid (21-aminosteroid) analogue U75412E was evaluated in rabbits exposed to diesel fuel-polycarbonate plastic smoke. Inhalation of a total of 4.6 mg U75412E aerosolized at a rate of 1.53 mg/min for 3 min before or after smoke significantly prevented or limited the extent of alveolar hypoventilation, interstitial edema, and tumor necrosis factor-α (TNF-α) by pulmonary alveolar macrophages (PAM) ex vivo observed at 2 h. The smoke-induced changes in wet lung/body weight ratios and the production of superoxide (O2− by PAM ex vivo were also attenuated by the drug treatment after smoke exposure (p ¼ 0.05). This study suggests that lazaroids may ameliorate the oxygen-radical-initiated cytokine processes and inflammation cascade as a result of the smoke insult.",

keywords = "Acute lung injury, Lazaroid, Pulmonary alveolar macrophages, Smoke, Superoxide, Tumor necrosis factor-andα",

author = "Shengjun Wang and Lantz, \{R. Clark\} and Rider, \{Evelyn D.\} and Chen, \{Guan Jie\} and Veronica Breceda and Hays, \{Allison M.\} and Robledo, \{Raymond F.\} and Tollinger, \{Brian J.\} and Dinesh, \{S. V.R.\} and Witten, \{Mark L.\}",

year = "1997",

month = jan,

day = "1",

doi = "10.1159/000196704",

language = "English (US)",

volume = "64",

pages = "358--363",

journal = "Respiration",

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TY - JOUR

T1 - A Free Radical Scavenger (Lazaroid U75412E) Attenuates Tumor Necrosis Factor-Alpha Generation in a Rabbit Model of Smoke-Induced Lung Injury

AU - Wang, Shengjun

AU - Lantz, R. Clark

AU - Rider, Evelyn D.

AU - Chen, Guan Jie

AU - Breceda, Veronica

AU - Hays, Allison M.

AU - Robledo, Raymond F.

AU - Tollinger, Brian J.

AU - Dinesh, S. V.R.

AU - Witten, Mark L.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The lazaroid (21-aminosteroid) analogue U75412E was evaluated in rabbits exposed to diesel fuel-polycarbonate plastic smoke. Inhalation of a total of 4.6 mg U75412E aerosolized at a rate of 1.53 mg/min for 3 min before or after smoke significantly prevented or limited the extent of alveolar hypoventilation, interstitial edema, and tumor necrosis factor-α (TNF-α) by pulmonary alveolar macrophages (PAM) ex vivo observed at 2 h. The smoke-induced changes in wet lung/body weight ratios and the production of superoxide (O2− by PAM ex vivo were also attenuated by the drug treatment after smoke exposure (p ¼ 0.05). This study suggests that lazaroids may ameliorate the oxygen-radical-initiated cytokine processes and inflammation cascade as a result of the smoke insult.

AB - The lazaroid (21-aminosteroid) analogue U75412E was evaluated in rabbits exposed to diesel fuel-polycarbonate plastic smoke. Inhalation of a total of 4.6 mg U75412E aerosolized at a rate of 1.53 mg/min for 3 min before or after smoke significantly prevented or limited the extent of alveolar hypoventilation, interstitial edema, and tumor necrosis factor-α (TNF-α) by pulmonary alveolar macrophages (PAM) ex vivo observed at 2 h. The smoke-induced changes in wet lung/body weight ratios and the production of superoxide (O2− by PAM ex vivo were also attenuated by the drug treatment after smoke exposure (p ¼ 0.05). This study suggests that lazaroids may ameliorate the oxygen-radical-initiated cytokine processes and inflammation cascade as a result of the smoke insult.

KW - Acute lung injury

KW - Lazaroid

KW - Pulmonary alveolar macrophages

KW - Smoke

KW - Superoxide

KW - Tumor necrosis factor-andα

UR - https://www.scopus.com/pages/publications/0030811224

UR - https://www.scopus.com/pages/publications/0030811224#tab=citedBy

U2 - 10.1159/000196704

DO - 10.1159/000196704

M3 - Article

C2 - 9311052

AN - SCOPUS:0030811224

SN - 0025-7931

VL - 64

SP - 358

EP - 363

JO - Respiration

JF - Respiration

IS - 5

ER -

A Free Radical Scavenger (Lazaroid U75412E) Attenuates Tumor Necrosis Factor-Alpha Generation in a Rabbit Model of Smoke-Induced Lung Injury

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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