A Free Radical Scavenger (Lazaroid U75412E) Attenuates Tumor Necrosis Factor-Alpha Generation in a Rabbit Model of Smoke-Induced Lung Injury

Shengjun Wang; R. Clark Lantz; Evelyn D. Rider; Guan Jie Chen; Veronica Breceda; Allison M. Hays; Raymond F. Robledo; Brian J. Tollinger; S. V.R. Dinesh; Mark L. Witten

doi:10.1159/000196704

A Free Radical Scavenger (Lazaroid U75412E) Attenuates Tumor Necrosis Factor-Alpha Generation in a Rabbit Model of Smoke-Induced Lung Injury

Shengjun Wang, R. Clark Lantz, Evelyn D. Rider, Guan Jie Chen, Veronica Breceda, Allison M. Hays, Raymond F. Robledo, Brian J. Tollinger, S. V.R. Dinesh, Mark L. Witten

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The lazaroid (21-aminosteroid) analogue U75412E was evaluated in rabbits exposed to diesel fuel-polycarbonate plastic smoke. Inhalation of a total of 4.6 mg U75412E aerosolized at a rate of 1.53 mg/min for 3 min before or after smoke significantly prevented or limited the extent of alveolar hypoventilation, interstitial edema, and tumor necrosis factor-α (TNF-α) by pulmonary alveolar macrophages (PAM) ex vivo observed at 2 h. The smoke-induced changes in wet lung/body weight ratios and the production of superoxide (O₂⁻ by PAM ex vivo were also attenuated by the drug treatment after smoke exposure (p ¼ 0.05). This study suggests that lazaroids may ameliorate the oxygen-radical-initiated cytokine processes and inflammation cascade as a result of the smoke insult.

Original language	English (US)
Pages (from-to)	358-363
Number of pages	6
Journal	Respiration
Volume	64
Issue number	5
DOIs	https://doi.org/10.1159/000196704
State	Published - Jan 1 1997
Externally published	Yes

Keywords

Acute lung injury
Lazaroid
Pulmonary alveolar macrophages
Smoke
Superoxide
Tumor necrosis factor-andα

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine

Access to Document

10.1159/000196704

Cite this

@article{efcbd5962d9143aaab0d879c6ed11022,

title = "A Free Radical Scavenger (Lazaroid U75412E) Attenuates Tumor Necrosis Factor-Alpha Generation in a Rabbit Model of Smoke-Induced Lung Injury",

abstract = "The lazaroid (21-aminosteroid) analogue U75412E was evaluated in rabbits exposed to diesel fuel-polycarbonate plastic smoke. Inhalation of a total of 4.6 mg U75412E aerosolized at a rate of 1.53 mg/min for 3 min before or after smoke significantly prevented or limited the extent of alveolar hypoventilation, interstitial edema, and tumor necrosis factor-α (TNF-α) by pulmonary alveolar macrophages (PAM) ex vivo observed at 2 h. The smoke-induced changes in wet lung/body weight ratios and the production of superoxide (O2− by PAM ex vivo were also attenuated by the drug treatment after smoke exposure (p ¼ 0.05). This study suggests that lazaroids may ameliorate the oxygen-radical-initiated cytokine processes and inflammation cascade as a result of the smoke insult.",

keywords = "Acute lung injury, Lazaroid, Pulmonary alveolar macrophages, Smoke, Superoxide, Tumor necrosis factor-andα",

author = "Shengjun Wang and Lantz, {R. Clark} and Rider, {Evelyn D.} and Chen, {Guan Jie} and Veronica Breceda and Hays, {Allison M.} and Robledo, {Raymond F.} and Tollinger, {Brian J.} and Dinesh, {S. V.R.} and Witten, {Mark L.}",

year = "1997",

month = jan,

day = "1",

doi = "10.1159/000196704",

language = "English (US)",

volume = "64",

pages = "358--363",

journal = "Respiration",

issn = "0025-7931",

publisher = "S. Karger AG",

number = "5",

}

TY - JOUR

T1 - A Free Radical Scavenger (Lazaroid U75412E) Attenuates Tumor Necrosis Factor-Alpha Generation in a Rabbit Model of Smoke-Induced Lung Injury

AU - Wang, Shengjun

AU - Lantz, R. Clark

AU - Rider, Evelyn D.

AU - Chen, Guan Jie

AU - Breceda, Veronica

AU - Hays, Allison M.

AU - Robledo, Raymond F.

AU - Tollinger, Brian J.

AU - Dinesh, S. V.R.

AU - Witten, Mark L.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The lazaroid (21-aminosteroid) analogue U75412E was evaluated in rabbits exposed to diesel fuel-polycarbonate plastic smoke. Inhalation of a total of 4.6 mg U75412E aerosolized at a rate of 1.53 mg/min for 3 min before or after smoke significantly prevented or limited the extent of alveolar hypoventilation, interstitial edema, and tumor necrosis factor-α (TNF-α) by pulmonary alveolar macrophages (PAM) ex vivo observed at 2 h. The smoke-induced changes in wet lung/body weight ratios and the production of superoxide (O2− by PAM ex vivo were also attenuated by the drug treatment after smoke exposure (p ¼ 0.05). This study suggests that lazaroids may ameliorate the oxygen-radical-initiated cytokine processes and inflammation cascade as a result of the smoke insult.

AB - The lazaroid (21-aminosteroid) analogue U75412E was evaluated in rabbits exposed to diesel fuel-polycarbonate plastic smoke. Inhalation of a total of 4.6 mg U75412E aerosolized at a rate of 1.53 mg/min for 3 min before or after smoke significantly prevented or limited the extent of alveolar hypoventilation, interstitial edema, and tumor necrosis factor-α (TNF-α) by pulmonary alveolar macrophages (PAM) ex vivo observed at 2 h. The smoke-induced changes in wet lung/body weight ratios and the production of superoxide (O2− by PAM ex vivo were also attenuated by the drug treatment after smoke exposure (p ¼ 0.05). This study suggests that lazaroids may ameliorate the oxygen-radical-initiated cytokine processes and inflammation cascade as a result of the smoke insult.

KW - Acute lung injury

KW - Lazaroid

KW - Pulmonary alveolar macrophages

KW - Smoke

KW - Superoxide

KW - Tumor necrosis factor-andα

UR - http://www.scopus.com/inward/record.url?scp=0030811224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030811224&partnerID=8YFLogxK

U2 - 10.1159/000196704

DO - 10.1159/000196704

M3 - Article

C2 - 9311052

AN - SCOPUS:0030811224

SN - 0025-7931

VL - 64

SP - 358

EP - 363

JO - Respiration

JF - Respiration

IS - 5

ER -

A Free Radical Scavenger (Lazaroid U75412E) Attenuates Tumor Necrosis Factor-Alpha Generation in a Rabbit Model of Smoke-Induced Lung Injury

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this