TY - JOUR
T1 - A divergent isolate of Tomato yellow leaf curl virus from Oman with an associated DNAβ satellite
T2 - An evolutionary link between Asian and the Middle Eastern virus-satellite complexes
AU - Khan, Akhtar Jamal
AU - Idris, Ali M.
AU - Al-Saady, Nadiya Abubaker
AU - Al-Mahruki, Madleen Said
AU - Al-Subhi, Ali Masoud
AU - Brown, J. K.
PY - 2008/2
Y1 - 2008/2
N2 - Tomato is cultivated in the coastal region of Al-Batinah, in the Sultanate of Oman, during the winter season, to meet the high demand for fresh produce in the domestic market. In order to identify the causal agent of a widespread disease associated with infestations of the whitefly Bemisia tabaci (Genn.) leaves were collected from tomato plants showing symptoms characteristic of the disease in Al-Batinah during 2004 and 2005. Total nucleic acids were isolated from the tomato leaves and used as the template for Φ29 DNA polymerase amplification of begomoviral circular DNA. Putative full unit length begomoviral DNA multimers were digested with Nco I and cloned into the plasmid vector pGEM7Zf+. The complete nucleotide (nt) sequence was determined as 2,765 bases, indicative of a monopartite begomoviral genome. A comparison of the genome sequence for the seven field isolates examined, indicated that they shared 99% nt identity. The virus from Oman was most closely related to TYLCV-IR at 91% nt identity, a monopartite begomoviral species described previously from Iran. Based on the guidelines of the ICTV the Oman isolate has been designated TYLCV-Om and is considered an isolate of TYLCV-IR. A satellite DNA (satDNA β), was amplified by polymerase chain reaction using degenerate primers and cloned, and the DNA sequence was determined. Analysis of the complete nt sequence of 1,371 bases indicated that the satDNA shared 88.5% similarity with its closest relatives, which are DNAβ molecules from tomato in Pakistan. This is the first report of a satDNA β associated with the TYLCV species. The TYLCV-Om and associated satDNA, thus represent a begomovirus-complex at the Asian-Middle East crossroads that quiet uniquely share geographical and genetic hallmarks of both.
AB - Tomato is cultivated in the coastal region of Al-Batinah, in the Sultanate of Oman, during the winter season, to meet the high demand for fresh produce in the domestic market. In order to identify the causal agent of a widespread disease associated with infestations of the whitefly Bemisia tabaci (Genn.) leaves were collected from tomato plants showing symptoms characteristic of the disease in Al-Batinah during 2004 and 2005. Total nucleic acids were isolated from the tomato leaves and used as the template for Φ29 DNA polymerase amplification of begomoviral circular DNA. Putative full unit length begomoviral DNA multimers were digested with Nco I and cloned into the plasmid vector pGEM7Zf+. The complete nucleotide (nt) sequence was determined as 2,765 bases, indicative of a monopartite begomoviral genome. A comparison of the genome sequence for the seven field isolates examined, indicated that they shared 99% nt identity. The virus from Oman was most closely related to TYLCV-IR at 91% nt identity, a monopartite begomoviral species described previously from Iran. Based on the guidelines of the ICTV the Oman isolate has been designated TYLCV-Om and is considered an isolate of TYLCV-IR. A satellite DNA (satDNA β), was amplified by polymerase chain reaction using degenerate primers and cloned, and the DNA sequence was determined. Analysis of the complete nt sequence of 1,371 bases indicated that the satDNA shared 88.5% similarity with its closest relatives, which are DNAβ molecules from tomato in Pakistan. This is the first report of a satDNA β associated with the TYLCV species. The TYLCV-Om and associated satDNA, thus represent a begomovirus-complex at the Asian-Middle East crossroads that quiet uniquely share geographical and genetic hallmarks of both.
KW - Begomovirus
KW - Bridge species
KW - DNA satellite
KW - Evolution
KW - Geminiviridae
KW - Tomato
UR - http://www.scopus.com/inward/record.url?scp=38849145788&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38849145788&partnerID=8YFLogxK
U2 - 10.1007/s11262-007-0163-3
DO - 10.1007/s11262-007-0163-3
M3 - Article
C2 - 17932737
AN - SCOPUS:38849145788
SN - 0920-8569
VL - 36
SP - 169
EP - 176
JO - Virus Genes
JF - Virus Genes
IS - 1
ER -