A chemotherapy-induced peripheral neuropathy model in Drosophila melanogaster

Research output: Chapter in Book/Report/Conference proceedingChapter


Peripheral neuropathies are one of the largest categories of neurodegenerative diseases. To investigate their mechanisms, many in vitro and in vivo models can be employed. Here we present a protocol for the induction of chemotherapy-induced peripheral neuropathy (CIPN) in the Drosophila melanogaster (fruit fly) model system. Using a clinically relevant degeneration initiator, paclitaxel (taxol), it is possible to model many aspects of axon and dendrite degeneration while in a genetically tractable, in vivo system. In this protocol, we feed larval stage Drosophila neurotoxic chemotherapy drugs during the duration of larval development, followed by dissection and imaging of genetically labeled sensory axons and dendrites. Both axons and dendrites degenerate with taxol exposure. Our protocol should facilitate the adoption and expansion of this model to include other neurotoxic compounds.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Axon
  • CIPN
  • Chemotherapy
  • Degeneration
  • Dendrite
  • Drosophila
  • Peripheral neuropathy
  • Sensory neuron
  • Taxol

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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