TY - JOUR
T1 - A century of guayule
T2 - Comprehensive genetic characterization of the US national guayule (Parthenium argentatum A. Gray) germplasm collection
AU - Ilut, Daniel C.
AU - Sanchez, Paul L.
AU - Coffelt, Terry A.
AU - Dyer, John M.
AU - Jenks, Matthew A.
AU - Gore, Michael A.
N1 - Funding Information:
We thank the National Arid Land Plant Genetics Resources Unit at Parlier, California for providing seeds. Also, we wish to thank Greg Leake and Brenda Singleton for maintaining plants, and Nicholas LaForest for leaf sample collection and ploidy analysis at the USDA-ARS, U.S. Arid-Land Agricultural Research Center in Maricopa, Arizona. Preparation of total genomic DNA samples for GBS was carried out by Nicholas Kaczmar in the Gore laboratory at Cornell University. We thank Olivia Tonge for assistance in a preliminary phylogenetic analysis of diploid guayule samples. This work was funded and supported by the USDA-ARS and USDA-NIFA/DOE Biomass Research and Development Initiative (BRDI) Grant No. 2012–10006. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. The USDA is an equal opportunity provider and employer.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/12/15
Y1 - 2017/12/15
N2 - The fragility of a single-source, geographically concentrated supply of natural rubber, a critical material of the modern economy, has brought guayule (Parthenium argentatum A. Gray) to the forefront as an alternative source of natural rubber. The improvement of guayule for commercial-scale production has been limited by the lack of genomic tools and well-characterized genetic resources required for genomics-assisted breeding. To address this issue, we developed nearly 50,000 single-nucleotide polymorphism (SNP) genetic markers and genotyped 69 accessions of guayule and its sister taxa mariola (Parthenium incanum Kunth), representing the entire publically available US national germplasm collection. We identified multiple interspecific hybrid accessions previously considered guayule, including five guayule-mariola hybrids and non-mariola interspecific hybrid accessions AZ-2 and AZ-3, two commonly used high-yielding cultivars. We dissected patterns of genetic diversity within the collection to identify a highly diverse subset of guayule accessions, and showed that wild guayule stands in Big Bend National Park, Texas, USA have the potential to provide hitherto untapped guayule genetic diversity. Together, these results provide the most thorough genetic characterization of guayule germplasm to date and lay the foundation for rapid genetic improvement of commercial guayule germplasm.
AB - The fragility of a single-source, geographically concentrated supply of natural rubber, a critical material of the modern economy, has brought guayule (Parthenium argentatum A. Gray) to the forefront as an alternative source of natural rubber. The improvement of guayule for commercial-scale production has been limited by the lack of genomic tools and well-characterized genetic resources required for genomics-assisted breeding. To address this issue, we developed nearly 50,000 single-nucleotide polymorphism (SNP) genetic markers and genotyped 69 accessions of guayule and its sister taxa mariola (Parthenium incanum Kunth), representing the entire publically available US national germplasm collection. We identified multiple interspecific hybrid accessions previously considered guayule, including five guayule-mariola hybrids and non-mariola interspecific hybrid accessions AZ-2 and AZ-3, two commonly used high-yielding cultivars. We dissected patterns of genetic diversity within the collection to identify a highly diverse subset of guayule accessions, and showed that wild guayule stands in Big Bend National Park, Texas, USA have the potential to provide hitherto untapped guayule genetic diversity. Together, these results provide the most thorough genetic characterization of guayule germplasm to date and lay the foundation for rapid genetic improvement of commercial guayule germplasm.
KW - Genotyping-by-sequencing
KW - Guayule
KW - Introgression
KW - Mariola
KW - Parthenium
KW - Polyploidy
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U2 - 10.1016/j.indcrop.2017.08.029
DO - 10.1016/j.indcrop.2017.08.029
M3 - Article
AN - SCOPUS:85028530784
SN - 0926-6690
VL - 109
SP - 300
EP - 309
JO - Industrial Crops and Products
JF - Industrial Crops and Products
ER -