A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia's north coast

Jahnier A. Caicedo; Gersson M. Vásquez; Claudia P. Calderón; Eliana Celis; Jennifer Natalie Castro; Joao Araujo-Junior; Hung N. Mai; Arun K. Dhar

doi:10.1016/j.jip.2025.108382

A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia's north coast

Jahnier A. Caicedo
, Gersson M. Vásquez
, Claudia P. Calderón
, Eliana Celis
, Jennifer Natalie Castro
, Joao Araujo-Junior
, Hung N. Mai
, Arun K. Dhar

Animal&Biomedical Sciences-Res

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

This study describes the diagnosis and reporting of IHHNV infection in two shrimp farms on Colombia's north coast in 2021. IHHNV was first detected in frozen Penaeus vannamei samples for export. Epidemiological investigation revealed IHHNV prevalence of 97 % and 36 % in the two farms using WOAH-recommended PCR. Histopathology showed no inclusion bodies, but in situ hybridization confirmed infection. Phylogenetic analysis identified the Colombian IHHNV isolates as genotype II, similar to strains from Ecuador, Brazil, and the US. These findings emphasize the importance of integrating molecular diagnostics, genome sequencing, and histopathology with in situ hybridization for comprehensive IHHNV detection and characterization.

Original language	English (US)
Article number	108382
Journal	Journal of Invertebrate Pathology
Volume	212
DOIs	https://doi.org/10.1016/j.jip.2025.108382
State	Published - Sep 2025

Keywords

Histopathology
IHHNV
In situ hybridization
PCR
Shrimp diseases

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

Access to Document

10.1016/j.jip.2025.108382

Fingerprint

Dive into the research topics of 'A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia's north coast'. Together they form a unique fingerprint.

Cite this

Caicedo, J. A., Vásquez, G. M., Calderón, C. P., Celis, E., Castro, J. N., Araujo-Junior, J., Mai, H. N., & Dhar, A. K. (2025). A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia's north coast. Journal of Invertebrate Pathology, 212, Article 108382. https://doi.org/10.1016/j.jip.2025.108382

A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia's north coast. / Caicedo, Jahnier A.; Vásquez, Gersson M.; Calderón, Claudia P. et al.
In: Journal of Invertebrate Pathology, Vol. 212, 108382, 09.2025.

Research output: Contribution to journal › Article › peer-review

Caicedo, JA, Vásquez, GM, Calderón, CP, Celis, E, Castro, JN, Araujo-Junior, J, Mai, HN & Dhar, AK 2025, 'A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia's north coast', Journal of Invertebrate Pathology, vol. 212, 108382. https://doi.org/10.1016/j.jip.2025.108382

Caicedo JA, Vásquez GM, Calderón CP, Celis E, Castro JN, Araujo-Junior J et al. A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia's north coast. Journal of Invertebrate Pathology. 2025 Sep;212:108382. doi: 10.1016/j.jip.2025.108382

@article{3ff88594d61e4a829ce480c542b99cc5,

title = "A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia's north coast",

abstract = "This study describes the diagnosis and reporting of IHHNV infection in two shrimp farms on Colombia's north coast in 2021. IHHNV was first detected in frozen Penaeus vannamei samples for export. Epidemiological investigation revealed IHHNV prevalence of 97 \% and 36 \% in the two farms using WOAH-recommended PCR. Histopathology showed no inclusion bodies, but in situ hybridization confirmed infection. Phylogenetic analysis identified the Colombian IHHNV isolates as genotype II, similar to strains from Ecuador, Brazil, and the US. These findings emphasize the importance of integrating molecular diagnostics, genome sequencing, and histopathology with in situ hybridization for comprehensive IHHNV detection and characterization.",

keywords = "Histopathology, IHHNV, In situ hybridization, PCR, Shrimp diseases",

author = "Caicedo, \{Jahnier A.\} and V{\'a}squez, \{Gersson M.\} and Calder{\'o}n, \{Claudia P.\} and Eliana Celis and Castro, \{Jennifer Natalie\} and Joao Araujo-Junior and Mai, \{Hung N.\} and Dhar, \{Arun K.\}",

note = "Publisher Copyright: {\textcopyright} 2025",

year = "2025",

month = sep,

doi = "10.1016/j.jip.2025.108382",

language = "English (US)",

volume = "212",

journal = "Journal of Invertebrate Pathology",

issn = "0022-2011",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia's north coast

AU - Caicedo, Jahnier A.

AU - Vásquez, Gersson M.

AU - Calderón, Claudia P.

AU - Celis, Eliana

AU - Castro, Jennifer Natalie

AU - Araujo-Junior, Joao

AU - Mai, Hung N.

AU - Dhar, Arun K.

PY - 2025/9

Y1 - 2025/9

N2 - This study describes the diagnosis and reporting of IHHNV infection in two shrimp farms on Colombia's north coast in 2021. IHHNV was first detected in frozen Penaeus vannamei samples for export. Epidemiological investigation revealed IHHNV prevalence of 97 % and 36 % in the two farms using WOAH-recommended PCR. Histopathology showed no inclusion bodies, but in situ hybridization confirmed infection. Phylogenetic analysis identified the Colombian IHHNV isolates as genotype II, similar to strains from Ecuador, Brazil, and the US. These findings emphasize the importance of integrating molecular diagnostics, genome sequencing, and histopathology with in situ hybridization for comprehensive IHHNV detection and characterization.

AB - This study describes the diagnosis and reporting of IHHNV infection in two shrimp farms on Colombia's north coast in 2021. IHHNV was first detected in frozen Penaeus vannamei samples for export. Epidemiological investigation revealed IHHNV prevalence of 97 % and 36 % in the two farms using WOAH-recommended PCR. Histopathology showed no inclusion bodies, but in situ hybridization confirmed infection. Phylogenetic analysis identified the Colombian IHHNV isolates as genotype II, similar to strains from Ecuador, Brazil, and the US. These findings emphasize the importance of integrating molecular diagnostics, genome sequencing, and histopathology with in situ hybridization for comprehensive IHHNV detection and characterization.

KW - Histopathology

KW - IHHNV

KW - In situ hybridization

KW - PCR

KW - Shrimp diseases

UR - https://www.scopus.com/pages/publications/105008539154

UR - https://www.scopus.com/pages/publications/105008539154#tab=citedBy

U2 - 10.1016/j.jip.2025.108382

DO - 10.1016/j.jip.2025.108382

M3 - Article

C2 - 40513982

AN - SCOPUS:105008539154

SN - 0022-2011

VL - 212

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

M1 - 108382

ER -

A case report about the detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp displaying no clinical signs or histopathological lesions from farms on Colombia's north coast

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this