4D display of the outflow track of embryonic-chick hearts (HH 14-19) using a high speed streak mode OCT

Siyu Ma, Rui Wang, Richard L. Goodwin, Roger R. Markwald, Thomas K. Borg, Raymond B. Runyan, Bruce Z. Gao

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Congenital Heart Disease (CHD) is the most common congenital malformation in newborns in the US. Although knowledge of CHD is limited, altered hemodynamic conditions are suspected as the factor that stimulates cardiovascular cell response, resulting in the heart morphology remodeling that ultimately causes CHDs. Therefore, one of recent efforts in CHD study is to develop high-speed imaging tools to correlate the rapidly changing hemodynamic condition and the morphological adaptations of an embryonic heart in vivo. We have developed a high-speed streak mode OCT that works at the center wavelength of 830 nm and is capable of providing images (292×220 μm2) of the outflow tract of an embryonic chick heart at the rate of 1000 Hz. The modality can provide a voxel resolution in the range of 10 μm3, and the spectral resolution allows a depth range of 1.63 mm. In the study reported here, each of the 4D images of an outflow tract was recorded for 2 seconds. The recording was conducted every 2 hours (HH17 to HH18), 3 hours (HH14 to HH17), and 4 hours (HH18 to HH19). Because of the fast scan speed, there is no need for postacquisition processing such as use of gating techniques to provide a fine 3D structure. In addition, more details of the outflow tract are preserved in the recorded images. The 4D images can be used in the future to determine the role of blood flow in CHD development.

Original languageEnglish (US)
Title of host publicationDynamics and Fluctuations in Biomedical Photonics IX
StatePublished - 2013
EventDynamics and Fluctuations in Biomedical Photonics VIII Conference - San Francisco, CA, United States
Duration: Feb 2 2013Feb 4 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherDynamics and Fluctuations in Biomedical Photonics VIII Conference
Country/TerritoryUnited States
CitySan Francisco, CA


  • CHDs
  • Embryonic chick heart
  • High speed imaging
  • Outflow tract
  • Streak mode OCT

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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