17β-Estradiol induction of filopodial growth in cultured hippocampal neurons within minutes of exposure

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Abstract

The rapid effect of 17β-estradiol upon filopodial growth was investigated using videomicroscopy of hippocampal neurons in culture. Within 1-10 min, 17β-estradiol induced a significant increase in the number of filopodia decorating neuritic extensions. In addition, the length of existing as well as newly formed filopodia increased significantly during the 10-min 17β-estradiol exposure. Neurite area and neurite length did not change significantly. The inactive epimer, 17β-estradiol, had no effect upon any of the morphological parameters assessed. Similarly, neither progesterone or corticosterone showed a significant influence upon any of the parameters of hippocampal nerve cell growth in culture within the same time frame in which 17β-estradiol effects were observed. Testosterone induced a significant increase in the number of filopodia without a significant increase in the overall length of the filopodia. Results of these studies demonstrate that 17β-estradiol can induce rapid changes in nerve cell morphology, suggesting that modification of neuronal circuitry can occur within minutes of exposure, a time base that is consistent with 17β-estradiol influence upon nerve cell excitability.

Original languageEnglish (US)
Pages (from-to)36-46
Number of pages11
JournalMolecular and Cellular Neurosciences
Volume4
Issue number1
DOIs
StatePublished - Feb 1993
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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